NCTC 2071

90050803, mouse connective tissue, Fibroblast

• UNSPSCコード: 41106514

特性

• 製品名: NCTC 2071, 90050803
• 由来生物: mouse connective tissue
• 成長モード: Adherent
• 核型: Modal no. 63
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse C3H/An connective tissue

細胞株の説明

Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.

アプリケーション

Nutritional, immunological and toxicological studies

培地

NCTC 135 100% + 2mM Glutamine.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm² by scraping off the cells and resuspending into new flasks; 5% CO₂; 37°C. Freeze in 9% glycerol/91% FBS.

その他情報

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