3T3(+3)

89022402, mouse unknown/unspecified, Fibroblast

• UNSPSCコード: 41106514

特性

• 製品名: 3T3(+3), NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 89022402
• 由来生物: mouse unknown/unspecified
• 包装: tube of 5 μg 89022402-DNA-5UG; pkg of vial of cells 89022402-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse

細胞株の説明

Please note: Available stocks of this cell line are not contact-inhibited. Contact inhibition is the natural process of arresting cell growth when cells come into contact with each other. It is not possible to be sure how the loss of contact inhibition may affect other characteristics of the cell line. This cell line catalog number continues to be a popular choice.

The key consideration is whether the characteristic of contact inhibition is required for cell line work. For example, it is required when testing the affects of expression of potential oncogenes where the induction of the loss of contact inhibition is being measured, whereas it is of less significance when using the cell line to express recombinant proteins for production and purification.

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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