SV3T3B

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSCコード: 41106514

特性

• 由来生物: mouse embryo
• 包装: tube of 5 μg 85011409-DNA-5UG; pkg of vial of cells 85011409-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse SIM embryo fibroblast

細胞株の説明

A 3T3 cell line doubly transformed by polyoma then SV40 viruses, probably the fore-runner of SV-T2 (Sigma Catalogue number. 90030801). Demonstrates a high degree of contact inhibition.

アプリケーション

Contact inhibition, transformation and virus studies: SV40

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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