MCF7/ExeR-4

16022523, human breast, Polygonal epithelia

• UNSPSCコード: 41106514

特性

• 製品名: MCF7/ExeR-4, from human breast, 16022523
• 由来生物: human breast
• 成長モード: Adherent
• 形態: Polygonal epithelia
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

詳細

Breast cancer cell line resistant to the aromatase inhibitor exemestane. Oestrogen receptor positive.

細胞株の説明

The MCF7/ExeR-4 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. Exemestane-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10^-7 M testosterone. A culture of MCF-7 cells were treated with 10^-7 M exemetane for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with exemestane. After ~2–3 months, the isolated colonies gave rise to exemestane-resistant cell lines, which could be grown in exemestane-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.

培地

Phenol red free DMEM:F12 (1:1) (D6434) + 10% Newborn Calf Serum(NBCS) (N4637) + 2.5 mM Ala-Gln (G8541) + 6 ng/ ml Insulin (I9278), 0.1 uM testosterone and 0.1 uM exemestane

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10, 000 cells/cm² using 0.05% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

引用

If use of this culture results in a scientific publication, it should be cited in the publication as: MCF7/ExeR-4 (ECACC 16022523)

免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.