C5585

モノクロナール抗ビオチン

clone BN-34, purified immunoglobulin, buffered aqueous solution

• 別名: Monoclonal Anti-Biotin
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: CY3 conjugate
• Clone: BN-34, monoclonal
• Application: IF, IHC (p)
• Citations: 7

特性

• biological source: mouse
• Quality Segment: 200
• conjugate: CY3 conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BN-34, monoclonal
• form: buffered aqueous solution
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, indirect immunofluorescence: 1:200
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

詳細

General description

競合ELISAを用いた場合、本抗体は遊離ビオチンおよびビオサイチンを認識します。間接ELISAまたは免疫組織化学染色を用いた場合、本抗体は、さまざまな免疫グロブリンに結合したビオチンも認識します。特異性は、マルチウェルプレートにコーティングされたヒトおよびウサギの抗原に対するビオチン化ヤギ抗体を使用して評価されました。

Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is a naturally occurring vitamin and co-enzyme required by cells in living organisms or in culture.

Immunogen

ビオチン-KLH結合体

Application

アビジンの代わりに使用して、非特異的な結合を低減させることができます。

本抗体は、増幅技術、ELISA、免疫ブロッティング、in situ核酸ハイブリダイゼーション、フローサイトメトリー、画像分析、または共焦点顕微鏡法に使用される場合があります。
一部のアプリケーションでは、アビジンによるビオチン化プローブの局在化により、バックグラウンドレベルが高くなります。非特異的結合を減らすために、抗ビオチン試薬がアビジンの代わりに使用される場合があります。

Monoclonal Anti-Biotin−Cy3^™ antibody produced in mouse has been used in:

• fluorescent microscopy
• RNA fluorescence in situ hybridization (FISH){36
• immunostaining

Biochem/physiol Actions

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.

Monoclonal Anti-Biotin- Cy3 can be used in many other applications where biotin can be introduced as target label. It can be used in detection of DNA and mRNA by sensitive non-isotopic in situ hybridization. It may also be used for detection of microinjected biotin-haptenized cytoskeletal proteins allowing examination of the pattern of incorporation and turnover of cytoskeletal proteins in living cells.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)