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由来生物
goat
結合体
alkaline phosphatase conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
buffered aqueous glycerol solution
テクニック
direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
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詳細
特異性と反応性は、ビオチンキャリアタンパク質複合体に対するオクタロニー二重拡散および免疫電気泳動によって決定します。キャリアタンパク質に対しての反応性は観察されません。
Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.
Goat Anti-Biotin-Alkaline Phosphatase antibody is specific for biotin when tested against biotin-goat IgG, goat IgG, and KLH.
Goat Anti-Biotin-Alkaline Phosphatase antibody is specific for biotin when tested against biotin-goat IgG, goat IgG, and KLH.
免疫原
ビオチン-KLH結合体
アプリケーション
方法によっては、アビジンとビオチン化プロ-ブの局在化によりバックグラウンド量が高くなることがあります。抗ビオチン試薬をアビジンの代わりに使用すると、非特異的な結合が低減できる場合があります。
ELISA Assays using human spleen cells were performed using alkaline phosphatase-conjugated goat anti-biotin as the secondary antibody with incubation for 1.5 hours at room temperature. Purified IgG from K/BxN, BxSB or C57BL/6 mouse serum was analyzed by western blot using alkaline phosphatase-conjugated goat anti-biotin antibody as the secondary.
Goat Anti-Biotin-Alkaline Phosphatase antibody has been used for dot blot assays. The product can also be used for ELISA (1:30,000) and immunohistochemistry (1:50).
物理的形状
0.05 M Tris溶液(pH 8.0, 10 mM グリシン, 1 mM MgCl2, 1%BSA, 50%グリセロ-ル, 15 mMアジ化ナトリウム含有)
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A7064-1ML:
A7064PROC:
A7064-VAR:
A7064-.25ML:
A7064-BULK:
A7064-.5ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Heike Albert et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(39), 15005-15009 (2008-09-26)
IgG antibodies are potent inducers of proinflammatory responses. During autoimmune diseases such as arthritis and systemic lupus erythematosus, IgG autoantibodies are responsible for the chronic inflammation and destruction of healthy tissues by cross-linking Fc receptors on innate immune effector cells.
Peter A Suci et al.
Chemistry & biology, 14(4), 387-398 (2007-04-28)
Nanomedicine directed at diagnosis and treatment of infections can benefit from innovations that have substantially increased the variety of available multifunctional nanoplatforms. Here, we targeted a spherical, icosahedral viral nanoplatform to a pathogenic, biofilm-forming bacterium, Staphylococcus aureus. Density of binding
Peter A Suci et al.
Langmuir : the ACS journal of surfaces and colloids, 22(21), 8891-8896 (2006-10-04)
Protein cage architectures such as viral capsids, heat shock proteins, ferritins, and DNA-binding proteins are nanoscale modular subunits that can be used to expand the structural and functional range of composite materials. Here, layer-by-layer (LbL) assembly was used to incorporate
Taiji Nozaki et al.
Journal of immunology (Baltimore, Md. : 1950), 179(8), 5238-5245 (2007-10-04)
Rejected MHC-mismatched cardiac allografts in CCR5(-/-) recipients have low T cell infiltration, but intense deposition of C3d in the large vessels and capillaries of the graft, characteristics of Ab-mediated rejection. The roles of donor-specific Ab and CD4 and CD8 T
Rocio Rodriguez-Melendez et al.
The Journal of nutritional biochemistry, 14(12), 680-690 (2003-12-24)
In mammals, biotin serves as coenzyme for four carboxylases, which play essential roles in the metabolism of glucose, amino acids, and fatty acids. Biotin deficiency causes decreased rates of cell proliferation, impaired immune function, and abnormal fetal development. Evidence is
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