The method of preparation is considered proprietary. However, as mentioned in this properties section of the Product Detail Page, this is Azurin from Pseudomonas aeruginosa. See the links below to review a sample Certificate of Analysis and the product data sheet, respectively:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A3672-1MG0000211689.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/100/a3672dat.pdf
A3672
アズリン
from Pseudomonas aeruginosa, lyophilized powder
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
1 mg | カートの在庫状況を確認する | ￥128,000 |
この商品について
Form:
lyophilized powder
Biological source:
Pseudomonas aeruginosa
biological source
Pseudomonas aeruginosa
Quality Segment
form
lyophilized powder
composition
Protein, ≥65% Lowry
concentration
≥65.0% (Lowry)
technique(s)
toxicology assay: suitable
solubility
water: soluble 1—1.1 mg/mL, clear, blue (light blue to blue)
UniProt accession no.
storage temp.
−20°C
Gene Information
Pseudomonas aeruginosa ... AZU(878046)
General description
Research area: Apoptosis. Azurinis a periplasmic protein and is a homotetramer.[1]
Application
Azurin has been used:
- in the cytotoxicity and cell viability studies in human osteosarcoma cell line
- for the functionalization of silicon nitride cantilevers for interaction studies
- for coating gold surface and insulating functionalized oxide surfaces of silicon oxide and mica
Biochem/physiol Actions
アズリンはクプレドキシンのファミリーに属する金属タンパク質です。正常細胞よりがん細胞に選択的に侵入し、アポトーシスを誘導します。アズリンはエフリンB2に構造的に類似しており、実際にエフリン受容体チロシンキナーゼEphB2に結合して、がん進展に関わる細胞シグナル伝達を開始させます。またEphB2チロシン残基の自己リン酸化を阻害し、上流の細胞シグナル伝達を阻害し、がん細胞の増殖抑制に寄与します。
Azurin acts as an electron donor for nitrite reductase in bacterial denitrification process.[1] It exhibits anticancer activity[1] as it hampers various independent signaling pathways associated with cancer progression. It binds to tumor suppressor protein p53 and induces cancer cell apoptosis or stalls cancer cell growth. Azurin disrupts angiogenesis by reducing the activity of VEGFR-2tyrosine kinase thereby inhibiting tumor growth. It has been observed to show cytotoxicity in human breast cancer cells and human melanoma cells.[2]
Physical form
酢酸アンモニウムバッファ-塩を含有する凍結乾燥粉末
1 of 1
当該品目 | |||
|---|---|---|---|
| biological source Pseudomonas aeruginosa | biological source - | biological source insect (firefly) | biological source Pseudomonas aeruginosa (10) |
| technique(s) toxicology assay: suitable | technique(s) toxicology assay: suitable | technique(s) toxicology assay: suitable | technique(s) - |
| concentration ≥65.0% (Lowry) | concentration - | concentration - | concentration - |
| form lyophilized powder | form powder | form solid | form lyophilized powder |
| Gene Information Pseudomonas aeruginosa ... AZU(878046) | Gene Information human ... CCK(885) | Gene Information - | Gene Information - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How do i know that this is azurin i mean any confirmatory test or anything else.
1 回答-
役に立ちましたか？
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