由来生物
human peripheral blood
リプログラミング法
Sendai virus
詳細
age (N/A)
メーカー／製品名
EBiSC™
性別
N/A
成長モード
adherent (pluripotent)
テクニック
cell culture | stem cell: suitable
関連疾患
Brugada syndrome
輸送温度
dry ice
保管温度
−196°C
詳細
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
細胞株の由来
Depositor
Klinikum der Universität zu Köln
Klinikum der Universität zu Köln
細胞株の説明
Derivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
TRA 1-80 Yes -
OCT 4 Yes - -
SSEA-4 Yes - -
NANOG Yes - -
DNMT3b Yes -
FOXD3 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
STEMdiff_Trilineage_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
TRA 1-80 Yes -
OCT 4 Yes - -
SSEA-4 Yes - -
NANOG Yes - -
DNMT3b Yes -
FOXD3 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
STEMdiff_Trilineage_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / Result
関連事項
Note: EAUA and CLIP must be completed before order fulfillment
継代と培養方法
Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 17
No. Vials banked (pre-EBiSC): 20
Passage method: Enzyme-free cell dissociation
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 17
No. Vials banked (pre-EBiSC): 20
法的情報
EBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies Corporation
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
劇物
Jan Code
66540877-1VL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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