由来生物
human dermis (fibroblast)
リプログラミング法
Sendai virus
詳細
age (55-59)
メーカー／製品名
EBiSC™
性別
female
成長モード
adherent (pluripotent)
テクニック
cell culture | stem cell: suitable
関連疾患
none
輸送温度
dry ice
保管温度
−196°C
詳細
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
細胞株の由来
Depositor
Wellcome Trust Sanger Institute
Wellcome Trust Sanger Institute
細胞株の説明
Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi046-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)NANOG(+)SOX2(+)
PluriTest: Pluripotency score: 38.268
Novelty score: 1.5
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123013
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi046-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)NANOG(+)SOX2(+)
PluriTest: Pluripotency score: 38.268
Novelty score: 1.5
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123013
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
関連事項
Note: EAUA and CLIP must be completed before order fulfillment
継代と培養方法
Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
CO2 concentration: 5%
Culture medium: TeSR® E8®
Passage Number banked (pre-EBiSC):
Passage method: Enzyme-free cell dissociation / EDTA
CO2 concentration: 5%
Culture medium: TeSR® E8®
Passage Number banked (pre-EBiSC):
法的情報
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
TeSR is a registered trademark of WiCell Research Institute, Inc.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
劇物
Jan Code
66540120-1VL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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