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05066

アガロース

High EEO

別名:

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE

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あなたへ/カタログ番号在庫状況単価
50 g
カートの在庫状況を確認する
¥29,000
500 g
カートの在庫状況を確認する
¥181,000

この商品について

CAS番号:
UNSPSC Code:
41105317
PubChem Substance ID:
EC Number:
232-731-8
NACRES:
NA.25
MDL number:

¥29,000


カートの在庫状況を確認する

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grade

Molecular Biology

Quality Segment

form

powder

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

ign. residue

≤1%

loss

≤10% loss on drying

EEO

0.23-0.27

transition temp

gel point 34-37 °C (1.5% solution)

gel strength

≥1500 g/cm2

anion traces

chloride (Cl-): ≤0.3%, sulfate (SO42-): ≤0.6%

cation traces

Ca: ≤0.05%, Cd: ≤0.001%, Co: ≤0.001%, Cr: ≤0.001%, Cu: ≤0.001%, Fe: ≤0.001%, K: ≤0.05%, Mg: ≤0.001%, Mn: ≤0.001%, Na: ≤0.5%, Ni: ≤0.001%, Pb: ≤0.001%, Zn: ≤0.001%

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

Application

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay.[1] It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.[2]

Biochem/physiol Actions

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage.[3] It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.[4]

Packaging

Packaging
50, 500 g in poly bottle

Analysis Note

以下は、アガロースに関する特性のリストです:
硫酸塩含量 - 硫酸塩は存在する主要なイオン基であるため、純度の指標として使用されます。
ゲル強度 - ゲルを破壊するためにゲルに加えなければならない力。
ゲル化点 - アガロース水溶液が冷えてゲルが生成する温度。アガロース溶液は、液体からゲルへの転移時にヒステリシスを示します。すなわち、それらのゲル化点は融解温度と同じではありません。
電気浸透(EEO)- ゲルの中を通り抜ける液体の動き。アガロースゲルのアニオン基はマトリックスに固定されて動けませんが、解離できるカウンターカチオンはマトリックスのカソードに向かって移動するため、EEOが起こります。通常、生体高分子の電気泳動の動きはアノードに向かうため、EEOは内部対流のために分離を乱す場合があります。

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当該品目
A5304A3643A6013
form

powder

form

powder

form

powder

form

powder

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

grade

Molecular Biology

grade

-

grade

-

grade

-

ign. residue

≤1%

ign. residue

-

ign. residue

-

ign. residue

-

cation traces

Ca: ≤0.05%, Co: ≤0.001%, Cu: ≤0.001%, K: ≤0.05%, Mn: ≤0.001%, Ni: ≤0.001%, Zn: ≤0.001%, Cd: ≤0.001%, Fe: ≤0.001%, Na: ≤0.5%, Cr: ≤0.001%, Pb: ≤0.001%, Mg: ≤0.001%

cation traces

-

cation traces

-

cation traces

-

impurities

DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected

impurities

≤11% moisture content

impurities

-

impurities

≤10% water


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保管分類

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Lot/Batch Number

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質問

1–2/2 質問  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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