由来生物
goat
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
クローン
polyclonal
形状
buffered aqueous solution
化学種の反応性
rabbit
濃度
~1 mg/mL
蛍光検出
λex 511 nm; λem 530 nm in PBS, pH 7.4
保管温度
−20°C
詳細
Abberior STAR 512 was developed for STED and confocal microscopy in the green spectral region. Abberior STAR 512 is highly photostable and bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation lasers at 488 nm or 514 nm. Abberior STAR 512 can substitute dyes like Oregon Green 514, Atto™ 514 or Alexa Fluor™ 514. For STED microscopy, Abberior STAR 512 can be most efficiently used with STED Laser wavelengths between 590 - 610 nm. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (PBS pH 7.4), 512 (H2O), 517 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 92 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,24 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 530 (PBS pH 7.4), 530 (H2O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (PBS pH 7.4), 512 (H2O), 517 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 92 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,24 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 530 (PBS pH 7.4), 530 (H2O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)
適合性
Designed and tested for fluorescent super-resolution microscopy
アナリシスノート
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
法的情報
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Gyuzel Yu Mitronova et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 16(15), 4477-4488 (2010-03-24)
New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to
S. M. Polyakova, et al.,
European Journal of Organic Chemistry, 30, 5162-5177 (2009)
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