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資料

39145

N-(7-Dimethylamino-4-methyl-3-coumarinyl)maleimide

Name: <I>N</I>-(7-Dimethylamino-4-methyl-3-coumarinyl)maleimide
Brand: SIAL

for fluorescence, ≥99.0% (HPLC)

別名:

DACM-3

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About This Item

実験式（ヒル表記法）:

C₁₆H₁₄N₂O₄

CAS番号:

55145-14-7

分子量:

298.29

Beilstein:

1545346

EC Number:

259-499-0

MDL番号:

MFCD00006863

UNSPSCコード:

12352200

PubChem Substance ID:

329755824

グレード

for fluorescence

アッセイ

≥99.0% (HPLC)

mp

218-220 °C (lit.)

蛍光検出

λ_ex 398 nm; λ_em 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)

SMILES記法

CN(C)c1ccc2C(C)=C(N3C(=O)C=CC3=O)C(=O)Oc2c1

InChI

1S/C16H14N2O4/c1-9-11-5-4-10(17(2)3)8-12(11)22-16(21)15(9)18-13(19)6-7-14(18)20/h4-8H,1-3H3

InChI Key

ADEORFBTPGKHRP-UHFFFAOYSA-N

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その他情報

タンパク質チオールに高い選択性を示す蛍光試薬で、ダンシルクロリドよりも優れた蛍光特性と水溶性を示します。

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number

352505/1

1144958

339956/1

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Y. Kanaoka

Angewandte Chemie (International Edition in English), 89, 141-141 (1977)

Use of hybridization for distance measurement by fluorescence energy transfer in oligomeric proteins: distance between two functional sites in aspartase.

S Murase et al.

Biochemical and biophysical research communications, 195(3), 1159-1164 (1993-09-30)

Our previous studies suggested that in a tetrameric enzyme aspartase, Cys-140 and Trp-430 are located at or near the catalytic and activator sites, respectively. To estimate the distance between these two sites, fluorescence energy transfer between a single tryptophan (Trp-430)

SH abundant body in infantile digital fibromatosis--a histochemical study on distribution of sulfhydryl groups.

M Kanamori et al.

The Journal of dermatology, 17(9), 559-563 (1990-09-01)

A case of infantile digital fibromatosis was studied with regard to the distribution of -SH groups and SS linkages, using N-(7-dimethylamino-4-methyl-3-coumarinyl) maleimide (DACM) stain. Tumor cells showed a strong fluorescence in the cytoplasm from the -SH stain. A brilliant -SH

Changes in SH reactivity of the protein in porcine intestinal brush-border membranes associated with lipid peroxidation.

T Ohyashiki et al.

Journal of biochemistry, 115(2), 224-229 (1994-02-01)

The effects of lipid peroxidation on the SH reactivity of the proteins in porcine intestinal brush-border membranes were examined using a fluorogenic thiol reagent, N-[7-dimethyl-amino-4-methylcoumarinyl]maleimide (DACM) in relation to lipid organization. Changes in the lipid organization were assessed by measurement

Trypanosoma cruzi phospho enol pyruvate carboxykinase (ATP-dependent): transition metal ion requirement for activity and sulfhydryl group reactivity.

L A Jurado et al.

Biochimica et biophysica acta, 1292(1), 188-196 (1996-01-04)

We studied the transition metal ion requirements for activity and sulfhydryl group reactivity in phospho enol pyruvate carboxykinase (PEP-carboxykinase; ATP:oxaloacetate carboxylase (transphosphorylating), EC 4.1.1.49), a key enzyme in the energy metabolism of the protozan parasite Trypanosoma (Schizotrypanum) cruzi. As for

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