由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
liquid
含まれません
preservative
交差性
rat, mouse, human
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
アイソタイプ
IgG
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
phosphorylation (pSer15)
遺伝子情報
human ... TP53(7157)
詳細
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.
Recognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
アプリケーション
Immunoblotting (1:1000)
Immunoprecipitation (1:200)
警告
Toxicity: Standard Handling (A)
物理的形状
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
再構成
Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
アナリシスノート
Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
その他情報
Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
PC386-50UL:
PC386-ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Timothy R Schwartz et al.
BMC molecular biology, 8, 7-7 (2007-01-30)
The repair of single base mutations in mammalian genes can be directed by single-stranded oligonucleotides in a process known as targeted gene repair. The mechanism of this reaction is currently being elucidated but likely involves a pairing step in which
Jasmine R Elison et al.
Archives of ophthalmology (Chicago, Ill. : 1960), 124(9), 1269-1275 (2006-09-13)
To determine the efficacy of inducing p53-mediated cell death in retinoblastoma cells by Nutlin 3A, a small molecule HDM2 inhibitor. Retinoblastoma cell lines WERI-RB-1 and Y79 were treated with Nutlin 3A. Cell viability assays, Western blot analyses, confocal microscopy, and
A-P Sappino et al.
Oncogenesis, 1, e7-e7 (2012-01-01)
The p53 tumor-suppressor protein has a key role in the induction of cellular senescence, an important barrier to cancer development. However, very little is known about the physiological mediators of cellular senescence induced by p53. CEACAM1 is an immunoglobulin superfamily
Rene Rodriguez et al.
Neoplasia (New York, N.Y.), 11(4), 397-407 (2009-03-25)
There is growing evidence about the role of mesenchymal stem cells (MSCs) as cancer stem cells in many sarcomas. Nevertheless, little is still known about the cellular and molecular mechanisms underlying MSCs transformation. We aimed at investigating the role of
André-Pascal Sappino et al.
Journal of applied toxicology : JAT, 32(3), 233-243 (2012-01-10)
Aluminium salts used as antiperspirants have been incriminated as contributing to breast cancer incidence in Western societies. To date, very little or no epidemiological or experimental data confirm or infirm this hypothesis. We report here that in MCF-10A human mammary
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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