MABT1335

Anti-Tropomyosin 3 Antibody, clone 2G10.2

• 別名: Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, TPM3, Tm3
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 2G10.2, monoclonal
• 分子量: ~30 kDa (Uncharacterized bands may be observed in some lysates.)
• 精製方法: using protein G
• 化学種の反応性: human, mouse, rat
• テクニック: immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG2bκ
• NCBIアクセッション番号: NP_705935
• UniProtアクセッション番号: Q63610
• 遺伝子情報: mouse ... TPM3(117557)

詳細

詳細

Tropomyosin alpha-3 chain (UniProt: Q63610; also known as Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5) is encoded by the Tpm3 (also known as Tpm-5, Tpm5) gene (Gene ID: 117557) in rat. The TPM3 gene codes for the slow-twitch skeletal muscle isoform ( s Tm) and at least 9 LMW cytoskeletal isoforms referred to as Tm5NM1 to Tm5NM11. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. In non-muscle cells tropomyosins are implicated in stabilizing cytoskeleton actin filaments. Tropomyosins have been implicated in the pathogenesis of cancer where high molecular weight isoforms are consistently down-regulated in transformed cells, while malignant cells display an increased reliance on low molecular weight isoforms. Tropomyosin 3 is a homodimeric protein that can form heterodimers with a beta (TPM2) chain. Its coiled coil structure is formed by 2 polypeptide chains. (Ref.: Glass, JJ et al. (2015). BMC Cancer. 15; 712).

特異性

Clone 2G10.2 is a mouse monoclonal antibody that specifically detects Tpm3.1 and Tpm3.2. It targets an epitope within 27 amino acids from the C-terminal region.

免疫原

Diphtheria Toxoid Carrier Protein-conjugated linear peptide corresponding to 27 amino acids from the C-terminal region of rat Tpm3.1 and Tpm3.2

アプリケーション

Western Blotting Analysis: 1 µg/mL from a representative lot detected Tropomyosin 3 in human lung tissue lysate.

Immunofluorescence Analysis: A representative lot detected Tropomyosin 3 in Immunofluorescence applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

Western Blotting Analysis: A representative lot detected Tropomyosin 3 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

品質

Evaluated by Western Blotting in mouse liver tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Tropomyosin 3 in mouse liver tissue lysate.

物理的形状

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.