MABS474

Anti-GRP78 Antibody, clone N88

clone N88, from mouse

• 別名: 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: N88, monoclonal
• 化学種の反応性: human, mouse
• テクニック: activity assay: suitable; flow cytometry: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_005338
• UniProtアクセッション番号: P11021
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HSPA5(3309)

詳細

詳細

The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER.

免疫原

Recombinant protein corresponding to human GRP78.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
神経シグナリング

Activity Assay Analysis: This antibody accelerates tumor growth in a B16F1 melanoma Flank tumor model (de Ridder, G. G., et al. (2012). Melanoma Research. 22(3):225-235).
Flow Cytometry Analysis: A representative lot detected GRP78 in B16F1 cells (de Ridder, G. G., et al. (2012). Melanoma Research. 22(3):225-235).

This Anti-GRP78 Antibody, clone N88 is validated for use in western blotting, activity assay & flow cytometry for the detection of GRP78.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GRP78 in 10 µg of HeLa cell lysate.

ターゲットの説明

~78 kDa observed

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable