MABS432

Anti-MEK1 Antibody, clone 6B7.1

clone 6B7.1, from mouse

• 別名: MAP kinase kinase 1, MEK1, MKK1, MAPKK 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 6B7.1, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_002746
• UniProtアクセッション番号: Q02750
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MAP2K1(5604)

詳細

詳細

Dual specificity mitogen-activated protein kinase kinase 1 (EC 2.7.12.2; UniProt Q02750; also known as ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1) is encoded by the MAP2K1 (also known as CFC3, MAPKK1, MEK1, MKK1, PRKMK1) gene (Gene ID 5604) in human. MEK1 and the related MEK2 are dual specificity kinases of the MAP2K/STE7 kinase family. MEK1 and MEK2 share a high degree of sequence homology and perform similar functions. Upon activation by RAF, MEK1/2 catalyze the dual phosphorylation of threonine and tyrosine residues on ERK (MAPK) within a specific Thr–Glu–Tyr sequence at codons 202 to 204. This locks the kinase domain of MAPK into a catalytically competent conformation, ultimately leading to nuclear signals that regulate cellular growth and proliferation. MEK1/2 mutations have been reported to be associated with cardiofaciocutaneous syndrome 3 (CFC3) and cancers, including lung cancer, colorectal cancer, and melanomas.

特異性

Expected to react with both the MKK1a and MKK1b spliced isoforms.

免疫原

Epitope: C-terminal region

GST-tagged recombinant protein corresponding to the C-terminal region of human MEK1.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
発生シグナル伝達

Anti-MEK1 Antibody, clone 6B7.1 is an antibody against MEK1 for use in Western Blotting.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MEK1 in 10 µg of HeLa cell lysate.

ターゲットの説明

~45 kDa observed

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable