由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
3A9E6, monoclonal
分子量
calculated mol wt 49.6 kDa
observed mol wt ~54 kDa
精製方法
using protein G
化学種の反応性
human
包装
antibody small pack of 100 μL
テクニック
ELISA: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
アイソタイプ
IgG1κ
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
輸送温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CLEC18A(348174)
詳細
C-type lectin domain family 18 member A (UniProt: A5D8T8; also known as Mannose receptor-like protein 2, CLEC18A) is encoded by the (also known as MRLP2) gene (Gene ID: 348174) in human. CLEC18A is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form (aa 27-446). Its expression is observed in all cells and in peripheral blood cells. It is mainly localized in the endoplasmic reticulum, Golgi apparatus, and endosome. Although it is expressed in normal hepatocytes, it is absent in hepatocellular carcinoma. It binds polysaccharides in a Ca2+-independent manner and shows preferential binding to fucoidal, b-glucans and galactans. It also serves as a co-receptor for TLR3 and contributes to the differential immune responses to poly(I:C) and H5N1 influenza A virus infection. Its levels are shown to be up-regulated upon differentiation of monocytes into macrophages and dendritic cells. Significantly higher levels of CLEC18A are observed subjects with hepatitis C infection and this increased level is associated with increased cryoglobulin levels, which may be related to the occurrence of mixed cryobulinemia (MC) syndrome. (Ref.: Huang, YL., et al. (2021). Nat. Commun. 4;229; Liao, TL., et al. (2018). Sci. Rep. 8; Article 17287; Huang, YL., et al. (2015). J. Biol. Chem. 290(35); 21252-21263).
特異性
Clone 3A9E6 is a mouse monoclonal antibody that detects human C-type lectin domain family 18 member A (CLEC18A).
免疫原
Purified recombinant human C-type lectin domain family 18 member A (CLEC18A) Fc fusion protein.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in U937 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CLEC18A in U937 cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63).
ELISA Analysis: A representative lot detected CLEC18A in ELISA applications (Liao T.L. et al. (2018). Sci Rep. 8(1):17287).
Western Blotting Analysis: A representative lot detected CLEC18A in Western Blotting applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Huang, Y.L, et al. (2021). Commun Biol. 4(1):229).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CLEC18A in human kidney tissue sections.
Flow Cytometry Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Liao T.L. et al. (2018). Sci Rep. 8(1):17287).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in U937 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CLEC18A in U937 cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63).
ELISA Analysis: A representative lot detected CLEC18A in ELISA applications (Liao T.L. et al. (2018). Sci Rep. 8(1):17287).
Western Blotting Analysis: A representative lot detected CLEC18A in Western Blotting applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Huang, Y.L, et al. (2021). Commun Biol. 4(1):229).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CLEC18A in human kidney tissue sections.
Flow Cytometry Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Liao T.L. et al. (2018). Sci Rep. 8(1):17287).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CLEC18A, clone 3A9E6, Cat. No. MABS2260, is a mouse monoclonal antibody that detects C-type lectin domain family 18 member A (CLEC18A) and is tested in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
物理的形状
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABS2260-100UL:
MABS2260-25UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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