MABS1268

Anti-MCRS1 Antibody, clone DON193B/C9

clone DON193B/C9, from rat

• 別名: Microspherule protein 1, 58 kDa microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: DON193B/C9, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_006328
• UniProtアクセッション番号: Q96EZ8
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MCRS1(10445)

詳細

詳細

Microspherule protein 1 (UniProt: Q96EZ8; also known as 58 kDa microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS1) is encoded by the MCRS1 (also known as INO80Q, MSP58) gene (Gene ID: 10445) in human. MCRS1 is a nucleolar protein that is detected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes. Its highest levels are reported in the early S phase and it becomes undetectable in the G2 phase. Four isoforms of MCRS1 have been reported that are produced by alternative splicing. MCRS1 contains a nucleolar localization motif (aa 113-123), a coiled-coil domain (aa 301-335), and a forkhead associated (FHA) domain (aa 363-419). MCRS1 exhibits transforming activity, which is suppressed by phosphatase and tensin homologue (PTEN). It can modulate the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 forms a complex with Mi-2 beta, and RET finger protein (RFP) and co-localize in the Nucleolus. This complex activates rRNA promoter activity. (Ref.: Shimono, K., et al. (2005). J. Biol. Chem. 280(47); 39436-39447).

特異性

Clone DON193B/C9 detects Microspherule protein 1 in human cells.

免疫原

A mixture of GST-tagged full length recombinant human Microspherule protein 1 and GST-tagged C- and N-terminal recombinant fragments of human Microspherule protein 1.

Epitope: unknown

アプリケーション

Research Category
細胞シグナル伝達

Anti-MCRS1, clone DON193B/C9, Cat. No. MABS1268, is a rat monoclonal antibody that detects Microspherule protein 1 and has been tested for use in Western Blotting.

Western Blotting Analysis: A representative lot detected MCRS1 in human tonsil, human spleen, HeLa cell, and Ramos cell lysates (Courtesy of Giovanna Roncador, Ph.D., CNIO, Madrid, Spain).

品質

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected MCRS1 in 10 µg of HCT116 cell lysate.

ターゲットの説明

~52 kDa observed; 51.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): does not flash
• 引火点(℃): does not flash