由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
197.2, monoclonal
化学種の反応性
human
テクニック
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... PF4(5196)
関連するカテゴリー
詳細
Platelet factor 4 (UniProt P02776; also known as C-X-C motif chemokine 4, Iroplact, Oncostatin-A, PF-4) is encoded by the PF4 (also known as CXCL4, SCYB4) gene (Gene ID 5196) in human. Platelet factor 4 (PF4) is a small CXC chemokine family cytokine released from alpha-granules of activated platelets during platelet aggregation to promote blood coagulation. PF4 binds heparin with high affinity (Kd ~4-20 nM) via its heparin-binding domain (a.a. 92-98) and neutralizes the anti-thrombin activity of heparin-like molecules. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with the chemokine receptor CXCR3 splice variant CXCR3B. In individuals suffering from heparin-induced thrombocytopenia (HIT), PF4-heparin complexes can trigger an immune response and the generation of anti-PF4 antibodies, resulting in the foramtion of macromolecular antibody-heparin-PF4 immune complexes that bind Fc RIIa (CD32a) on platelets and induce platelet activation. Platelet activation in turn results in more PF4 release into circulation, enhanced immune-complex formation, exaggerated platelet activation, and cell clearance. PF4 is synthesized with a signal peptide sequence (a.a. 1-31) that is removed posttranslationally to produce the mature PF4 (a.a. 32-101), additional proteolytic cleavage yields the PF4 short form (a.a. 48-101).
特異性
Clone 197.2 targets a linear epitope sensitive to heparin binding, but insensitive to reducing agent or denaturation by SDS (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
免疫原
Purified human platelet PF4 (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
アプリケーション
Detect PF4 using this mouse monoclonal Anti-Platelet Factor 4, clone 197.2, Cat. No. MABS1255, validated for use in ELISA, Flow Cytometry, Function Analysis, Immunocytochemistry. and Western Blotting.
Western Blotting Analysis: A representative lot detected E. coli expressed recombinant mature human PF4 (a.a. 32-101) under both reduced and non-reduced condition (Courtesy of Dr. Richard H. Aster, MD, PhD, Blood Center of Wisconsin, U. S. A.).
ELISA Analysis: A representative lot detected PF4 in the absence of heparin by ELISA. Decreased immunoreactivity of clone 197.2 was seen toward heparin-complexed PF4 (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Function Analysis: A representative lot and PF4, but not clone 197.2 or PF4 alone, activated human neutrophils as indicated by Mac-1 upregulation (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Immunocytochemistry Analysis: A representative lot, preconjugated with Alexa Fluor™ 488, immunolabeled the co-administered PF4 on human neutrophils. CD32a (Fc gamma RIIA) was seen co-localized with the PF4 anti-PF4 immune complexes on the surface of activated neutrophils (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Western Blotting Analysis: A representative lot detected PF4 under both reduced and non-reduced condition by recognizing a linear epitope (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
ELISA Analysis: A representative lot detected PF4 in the absence of heparin by ELISA. Decreased immunoreactivity of clone 197.2 was seen toward heparin-complexed PF4 (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Function Analysis: A representative lot and PF4, but not clone 197.2 or PF4 alone, activated human neutrophils as indicated by Mac-1 upregulation (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Immunocytochemistry Analysis: A representative lot, preconjugated with Alexa Fluor™ 488, immunolabeled the co-administered PF4 on human neutrophils. CD32a (Fc gamma RIIA) was seen co-localized with the PF4 anti-PF4 immune complexes on the surface of activated neutrophils (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Western Blotting Analysis: A representative lot detected PF4 under both reduced and non-reduced condition by recognizing a linear epitope (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
品質
Evaluated by Flow Cytometry in human platelets.
Flow Cytometry Analysis: 1 µg of this antibody detected PF4 in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized human platelets.
Flow Cytometry Analysis: 1 µg of this antibody detected PF4 in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized human platelets.
ターゲットの説明
~8 kDa (recombinant mature hPF4) observed. 10.84 kDa (pro-form), 7.769 kDa (mature; a.a. 32-101), 6.033 kDa (cleaved short form; a.a. 48-101) calculated. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Format: Purified
Purified mouse IgG1 in buffer containing PBS without preservatives.
その他情報
Concentration: Please refer to lot specific datasheet.
法的情報
ALEXA FLUOR is a trademark of Life Technologies
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABS1255:
試験成績書（COA）
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