MABS1229

Anti-SKP1 Antibody, clone 3F3.1

clone 3F3.1, from mouse

• 別名: S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, OCP-2, OCP-II, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polype
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 3F3.1, monoclonal
• 化学種の反応性: mouse, human
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_008861
• UniProtアクセッション番号: P63208
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SKP1(6500)

詳細

詳細

S-phase kinase-associated protein 1 (UniProt P63208; also known as Cyclin-A/CDK2-associated protein p19, OCP-2, OCP-II, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like) is encoded by the SKP1 (also known as EMC19, OCP2, SKP1A, TCEB1L) gene (Gene ID 6500) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Skp1 is one of the three core components of the SCF complex (Skp1, Cullin, F-box) and functions as an adapter that bridges the F-box protein and Cullin (CUL1). SCF complex depends on various F-box proteins for specific recognition and recruitment of substrates. SCF ligase plays an important role in regulating the transitions between G1/S and G2/M phases.

特異性

Expected to react with both spliced isoforms of human SKP1 reported by UniProt (P63208).

免疫原

Epitope: Internal.

GST-tagged recombinant human SKP1 internal fragment.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
ユビキチン及びユビキチン代謝

Anti-SKP1 Antibody, clone 3F3.1 is an antibody against SKP1 for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected SKP1 in HUVECs, A431 and HeLa cells.

品質

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SKP1 in 10 µg of NIH/3T3 cell lysate.

ターゲットの説明

~18 kDa observed. 18.66 kDa (Isoform 1) and 18.06 kDa (Isoform 2) calculated.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable