由来生物
mouse
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
11E7.1, monoclonal
化学種の反応性
mouse, human
テクニック
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CABP1(9478)
詳細
Calcium-binding protein 1 (UniProt Q9NZU7; also known as CaBP1, Calbrain, Caldendrin) is encoded by the CABP1 gene (Gene ID 9478) in human. Caldendrin is the founding member of the nCaBP family of neuronal Ca2+-inding proteins, members of which also include CaBP2 through CaBP8. nCaBPs and neuronal calcium sensor (NCS) family members are EF-hand-type calmodulin- (CaM-) like Ca2+-sensor proteins that play important roles in neuronal Ca2+-signaling. In addition to Caldendrin, the CABP1 gene produces three other alternative splicing isoforms, L-CaBP1/Caldendrin-S2, S-CaBP1/Caldendrin-S1, and Calbrain, with varying truncated N-terminal sequence. Caldendrin, L- and S-CaBP1 share four common EF-hands, while the Calbrain isoform contains only the third and fourth EF-hands and lacks the first two. EF-hand 1 constitutively binds Mg2+ and EF-hands 3 and 4 mediate high affinity Ca2+ binding, whereas EF-hand 2 does not bind either Mg2+ or Ca2+. In addition, Caldendrin lacks the N-myristoylation sequence present in L- and S-CaBP1 and many NCS proteins. The unique Caldendrin N-terminal sequence not found in the other spliced isoforms is bipartite in nature, composed of a highly basic proline-rich N-terminal and an acidic C-terminal part. Caldendrin and S-CaBP1 are reported to interact with the L-type voltage-gated calcium channel (VGCC) Cav1.2 via different molecular determinants, and dispaly different effects on Cav1.2 channel activity.
特異性
Clone 11E7.1 recognizes an internal epitope in spliced isoform Caldendrin (UniProt Q9NZU7-4). The exact location of the epitope has not been mapped. Although not confirmed by direct testing, this monoclonal antibody most likely also reacts with spliced isoform L-CaBP1 (Caldendrin-S2; UniProt Q9NZU7-1), while cross-reactivity toward isoform S-CaBP1 (Caldendrin-S1; UniProt Q9NZU7-2) and isoform Calbrain (UniProt Q9NZU7-3) is unlikely.
免疫原
GST-tagged recombinant human CaBP1 internal fragment.
アプリケーション
Immunohistochemistry Analysis: An 1:250 dilution from a representative lot detected CaBP1 in human cerebellum and human retina tissue.
This Anti-CaBP1 Antibody, clone 11E7.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CaBP1.
品質
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CaBP1 in 10 µg of human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CaBP1 in 10 µg of human brain tissue lysate.
ターゲットの説明
~40 kDa observed. 39.8 kDa (isoform Caldendrin), 25.0 kDa (Isoform L-CaBP1; Caldendrin-S2), 19.4 kDa (Isoform S-CaBP1; Caldendrin-S1), 8.14 kDa (Isoform Calbrain) calculated.
物理的形状
Format: Purified
その他情報
Concentration: Please refer to lot specific datasheet.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABN849:
試験成績書（COA）
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