由来生物
mouse
品質水準
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
3H9, monoclonal
交差性
rat, human
テクニック
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SMAD5(4090)
関連するカテゴリー
詳細
SMAD5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5 encoded by the gene named SMAD5 or MADH5 is a transcriptional cofactor activated by BMP type 1 receptor kinase and like other SMAD family members SMAD5 belongs to the larger TGFbeta superfamily of signaling modulators. SMAD5 forms complexes with SMAD4 when phosphorylated by BMPR1 and migrates to the nucleus. In the absence of phosphorylation SMAD5 is cytoplasmically localized. SMAD5 levels are regulated via ubiquitin mediated proteolysis facilitated by the E3 ligase SMURF1. SMAD5 is involved in many developmental pathways including in hematopoietic cells where SMAD5 activity is involved in the inhibition of the proliferation of hematopoietic progenitor cells. More recent research has shown SMAD5 to be involved in many other pathways too, for instance homozygous mutants die early in development and display a variable phenotype. Moreover SMAD5 is critical for proper granule cell development in the cerebellum. Finally because SMAD5 belongs to the TGFbeta family whose normal function is usually growth suppression, SMAD5 is often mutated in many cancers and its expression is associated with many forms of cancers in a similar fashion to that of its other family members. EMD-Millipore’s Anti-SMAD5 mouse monoclonal antibody has been tested in western blot on HeLa, SK-N-SH, PC-12 , Jurkat , and K562 cell lysates and in paraffin embedded immunohistochemistry on human brain and lung cancer tissue sections and in fluorescent immunocytochemistry on NTERA-2 cells in culture and in flow cytometry on Jurkat cells in culture.
免疫原
Purified recombinant fragment of human SMAD5 expressed in E. Coli.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経シグナリング
神経シグナリング
Detect Smad using this mouse monoclonal antibody, Anti-SMAD5 Antibody, clone 3H9 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected SMAD5 in NTERA-2 cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SMAD5 in human brain and lung cancer tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SMAD5 in Jurkat cells.
Optimal working dilutions must be determined by end user.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SMAD5 in human brain and lung cancer tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SMAD5 in Jurkat cells.
Optimal working dilutions must be determined by end user.
品質
Evaluated by Western Blotting in HeLa, SK-N-SH, PC-12, Jurkat, and K562 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SMAD5 in HeLa, SK-N-SH, PC-12, Jurkat, and K562 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SMAD5 in HeLa, SK-N-SH, PC-12, Jurkat, and K562 cell lysates.
ターゲットの説明
~52 kDa observed. Uncharacterized bands may appear in some lysate(s).
物理的形状
Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
HeLa, SK-N-SH, PC-12, Jurkat, and K562 cell lysates
HeLa, SK-N-SH, PC-12, Jurkat, and K562 cell lysates
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABN718:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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