由来生物
hamster
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
H24G11, monoclonal
分子量
calculated mol wt 70.33 kDa
observed mol wt ~85 kDa
精製方法
using protein G
化学種の反応性
mouse
包装
antibody small pack of 100 μL
テクニック
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
アイソタイプ
IgGκ
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
輸送温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Crtac1(72832)
詳細
Cartilage acidic protein 1 (UniProt: Q8R555; also known as 68 kDa chondrocyte-expressed protein, CEP-68, ASPIC, Protein CRTAC1-B, Lateral olfactory tract usher substance, LOTUS) is encoded by the Crtac1 (also known as Aspic1, Cep68) gene (Gene ID: 72832) in murine species. LOTUS is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form (aa 29-646). It is a membrane-bound and/or secreted protein that serves as an endogenous Nogo receptor-1 (NgR1) antagonist. It is shown to contribute to the formation of the lateral olfactory tract (LOT) axonal bundle. Endogenous Nogo-A signaling through NgR1 is shown to induce axonal branching. Developmental up-regulation of Nogo-A promotes collateral formation of the LOT during the late development period. It has been reported that axon growth inhibitors, which include Nogo proteins, myelin-associated glycoprotein (MAG), oligodendrocyte myelin glycoprotein (OMgp), and B lymphocyte stimulator (BLyS), bind to NgR1 and restrict functional recovery after damage to the adult central nervous system. LOTUS blocks the binding of these ligands to NgR1 and suppresses growth cone collapse. LOTUS knockout mice show significantly increased collateral branches of axons in the LOT. On the other hand, this branching is diminished in NgR1 knockout mice. A simultaneous loss of LOTUS and NgR1 is reported to rescue the abnormal increase in axonal branches. (Ref.: Iketani, M., et al. (2016). Sci. Rep. 6; 39586; Kurihara, Y., et al. (2014). Mol. Cell. Neurosci. 61; 211-218).
特異性
Clone H24G11 is a hamster monoclonal antibody that detects murine cartilage acidic protein 1B (LOTUS).
免疫原
Homogenate of protein extract from developing lateral olfactory tract and the surrounding tissues of mouse.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in Mouse E14 brain tissue lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LOTUS in Mouse E14 brain tissue lysates.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected LOTUS in Immunocytochemistry applications (Kurihara, Y., et al. (2014). Mol Cell Neurosci. 61:211-8; Sato, Y., et al. (2011). Science. 333(6043):769-73).
Immunohistochemistry Applications: A 1:50 dilutions from a representative lot detected LOTUS in E14 embryonic mouse tissue sections.
Western Blotting Analysis: A representative lot detected LOTUS in Western Blotting applications (Iketani, M., et al. (2016). Sci Rep. 6:39586).
Immunohistochemistry Applications: A representative lot detected LOTUS in Immunohistochemistry applications ( Sato, Y., et al. (2011). Science. 333(6043):769-73; Iketani, M., et al. (2016). Sci Rep. 6:39586).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Mouse E14 brain tissue lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LOTUS in Mouse E14 brain tissue lysates.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected LOTUS in Immunocytochemistry applications (Kurihara, Y., et al. (2014). Mol Cell Neurosci. 61:211-8; Sato, Y., et al. (2011). Science. 333(6043):769-73).
Immunohistochemistry Applications: A 1:50 dilutions from a representative lot detected LOTUS in E14 embryonic mouse tissue sections.
Western Blotting Analysis: A representative lot detected LOTUS in Western Blotting applications (Iketani, M., et al. (2016). Sci Rep. 6:39586).
Immunohistochemistry Applications: A representative lot detected LOTUS in Immunohistochemistry applications ( Sato, Y., et al. (2011). Science. 333(6043):769-73; Iketani, M., et al. (2016). Sci Rep. 6:39586).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-LOTUS, clone H24G11, Cat. No. MABN2614, is a hamster monoclonal antibody that detects Cartilage acidic protein 1 (LOTUS) and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
物理的形状
Purified hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABN2614-25UL:
MABN2614-100UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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