MABF356

Anti-CD45RO (human) Antibody, FITC, clone UCHL1

clone UCHL1, from mouse, FITC conjugate

• 別名: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, CD45RO
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

特性

• 由来生物: mouse
• 結合体: FITC conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: UCHL1, monoclonal
• 化学種の反応性: human
• テクニック: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• UniProtアクセッション番号: P08575
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PTPRC(5788)

詳細

詳細

Receptor-type tyrosine-protein phosphatase C, also known as Leukocyte common antigen (L-CA) or CD45 and encoded by the human gene PTPRC or CD45 is a transmembrane glycoprotein that belongs to the PTP family. PTPs are signaling molecules that regulate cellular process like cell growth, differentiation and mitotic cycle. CD45 has many isoforms that are distributed throughout the immune system according to cell type. These arise because of alternative splicing of RA, RB, and RC exons. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45RO is one of the isoforms of CD45 that facilitates T cell activation. It is also the shortest isform which lack the RA, RB, and RC exons. Mutations in CD45 have been associated with mulitple sclerosis and severe combined immunodeficiency.

特異性

This antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells.

免疫原

Cultured T cells from an IL-2 dependent T-cell line (CA1) prepared from human peripheral blood.

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
サイトカイン及びサイトカイン受容体

Anti-CD45RO (human) Antibody, FITC, clone UCHL1 is a highly specific mouse monoclonal antibody, that targets CD45RO & has been tested in Flow Cytometry, western blotting, IHC & IP.

Western Blotting Analysis: A representative lot detected human CD45RO in Western Blotting. (Ishii T, et al. 2001.)

Immunohistochemistry Analysis: A representative lot detected human CD45RO in Immunohistochemistry. (Yamada M, et al.1996.;Sakkas LI, et al. 1998.)

Immunoprecipitation Analysis: A representative lot detected human CD45RO in Immunoprecipitation. (Ponsford M, et al. 2001.)

品質

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.

Flow Cytometry Analysis: 20 μL of this antibody detected CD45RO in one million human peripheral blood lymphocytes.

ターゲットの説明

147 kDa calculated

物理的形状

Affinity

Purified mouse conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide and 0.2% (w/v) BSA.

保管および安定性

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable