MABF3063

Anti-Tax-1 Antibody, clone Lt-4

• 別名: Protein PX, Protein Tax-1, Protein X-LOR, Trans-activating transcriptional regulatory protein of HTLV-1
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: Lt-4, monoclonal
• 分子量: calculated mol wt 39.47 kDa; observed mol wt ~60 kDa
• 精製方法: using protein G
• 化学種の反応性: (T-cell leukemia virus 1 (HTLV-1))
• 包装: antibody small pack of 100
• テクニック: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG3κ
• エピトープ配列: Internal
• タンパク質IDアクセッション番号: N/A
• UniProtアクセッション番号: P03409
• 保管温度: 2-8°C

詳細

特異性

Clone Lt-4 is a mouse monoclonal antibody that detects Protein Tax-1.

免疫原

Partially purified Tax-1 protein preparation from HTLV-1 infected TCL-Kan cells

アプリケーション

Quality Control Testing

Evaluated by Western Blotting with recombinant Human T-cell leukemia virus 1 Protein Tax-1.

Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant Human T-cell leukemia virus 1 Protein Tax-1.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Protein Tax-1 in Flow Cytometry applications (Peres, C., et al. (2019). J Virol Methods. 274:113728).

ELISA: A representative lot detected Protein Tax-1 in ELISA applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Protein Tax-1 in Immunoprecipitation applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

Western Blotting Analysis: A representative lot detected Protein Tax-1in Western Blotting applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11; Tanaka, Y., et al. (1991). Int J Cancer. 48(4):623-30).

Immunocytochemistry Analysis: A representative lot detected Protein Tax-1 in Immunocytochemistry applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Protein Tax-1 (UniProt: P03409; also known as Protein X-LOR, Protein PX, Trans-activating transcriptional regulatory protein of HTLV-1) is encoded by the Tax gene in human. Tax-1 is a homodimeric protein that serves as a transcriptional activator that governs the viral transcription from the 5′LTR via the recruitment of dimers of host phosphorylated CREB1. Together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription. It is reported to shuttle from the host nucleus to the cytoplasm and is found predominantly in the nucleus, where it is equally distributed between the nucleoplasm and the nuclear matrix. Tax-1 is reported to modulate the expression of cellular genes leading to the deregulation of T-cell proliferation, disturbing the integrity of cell cycle checkpoints, the DNA damage response, and apoptotic pathways. It is also shown to act as a ubiquitin E3 ligase and stimulates host IKK complex by catalyzing the assembly of free mixed-linkage polyubiquitin chains, resulting in constitutive activation of NF-kB. Phosphorylation of Tax-1 on threonine 48 is shown to result in the loss of NF-kB activation function. Clone Lt-4 recognizes the Tax-1 protein expressed in HTLV-I-infected cells but does not react with uninfected cell lines and fresh and PHA-activated peripheral blood lymphocytes. (Ref.: Peres, C., et al. (2019). J. Virol. Methods. 274; 113728; Wang C., et al. (2016). PLoS Pathog. 12(4); e1005584; Tie, F., et al (1996). J. Virol. 70(12); 8368-8374; Tanaka, Y., et al. (1991). Int J Cancer. 48(4); 623-630; Lee, B., et al. (1989). Tohoku J. Exp. Med. 157(1); 1-11).

物理的形状

Purified mouse monoclonal antibod yIgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

再構成

0.5 mg/ml. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable