おすすめの製品
由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
MH9A3, monoclonal
分子量
calculated mol wt 15.91 kDa
精製方法
using protein G
化学種の反応性
human
包装
antibody small pack of 100 μL
テクニック
ELISA: suitable
flow cytometry: suitable
multiplexing: suitable
neutralization: suitable
アイソタイプ
IgG1
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... il9> IL9(3578)
詳細
Interleukin-9 (UniProt: P15248; also known as IL-9, Cytokine P40, T-cell growth factor P40) is encoded by the IL9 gene (Gene ID: 3578) in human. IL-9 is a pleiotropic cytokine that plays a significant role in tolerance and inflammation. It is expressed by a variety of immune cells. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form. It supports the growth of mast cells, T cells, and several other cell types, including erythroid progenitors, fetal thymocytes, human megakaryoblastic leukemic cells, and myeloid precursor cells. It is also reported to supports IL-2 independent and IL-4 independent growth of helper T-cells. It also plays a role in promoting IgE production by B cells. IL-9 actions are mediated via its binding to IL-9R that results in the formation the IL-9R heterocomplex. Following its binding to the IL-9R , the SHC is phosphorylated, which then binds to Grb2 that subsequently activates SoS, a GTP exchange factor for Ras GTPases. This results in the activation of Ras GTPase that activates Raf kinase followed by the activation of MEK1/2 and ERK1/2. Although IL-9 is reported to activate the MAPK pathway in several lymphoid and hematopoietic cells, this activation is weaker compared to other cytokines. Elevated levels of IL-9 and IL-9R are observed in lungs of asthmatic subjects. (Ref.: Chakraborty, S., et al. (2019). Int. J. Mol. Sci. 20(9); 2113).
特異性
Clone MH9A3 is a mouse monoclonal antibody that detects human Interleukin-9.
免疫原
Ovalbumin-conjugated recombinant human Interleukin-9.
アプリケーション
Quality Control Testing
Evaluated by ELISA with recombinant human IL-9.
ELISA Analysis: Various dilution of this antibody detected recombinant human Interleukin-9 (IL-9).
Tested Applications
ELISA Analysis: A representative lot detected IL-9 in ELISA applications (Jenmalm, M.C., et al. (2001). Clin Exp Allergy.;31(10):1528-35; Faulkner, H., et al. (2002). J Infect Dis.;185(5):665-72; Bottcher, M.F., et al. (2003). Pediatr Allergy Immunol.;14(5):345-50; Holz, L.E., et al. (2005). J Inflamm (Lond).;2:3.; Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80; Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).
Flow Cytometry Analysis: A representative lot detected IL-9 in Flow Cytometry applications (Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).
Multiplexing: A representative lot detected IL-9 in Multiplexing applications (Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).
Neutralizing: A representative lot neutralized the action of IL-9 in Neutralizing applications (Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by ELISA with recombinant human IL-9.
ELISA Analysis: Various dilution of this antibody detected recombinant human Interleukin-9 (IL-9).
Tested Applications
ELISA Analysis: A representative lot detected IL-9 in ELISA applications (Jenmalm, M.C., et al. (2001). Clin Exp Allergy.;31(10):1528-35; Faulkner, H., et al. (2002). J Infect Dis.;185(5):665-72; Bottcher, M.F., et al. (2003). Pediatr Allergy Immunol.;14(5):345-50; Holz, L.E., et al. (2005). J Inflamm (Lond).;2:3.; Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80; Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).
Flow Cytometry Analysis: A representative lot detected IL-9 in Flow Cytometry applications (Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).
Multiplexing: A representative lot detected IL-9 in Multiplexing applications (Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).
Neutralizing: A representative lot neutralized the action of IL-9 in Neutralizing applications (Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-9, clone MH9A3, Cat. No. MABF2786, is a mouse monoclonal antibody that detects Interleukin-9 and is tested for use in ELISA, Flow Cytometry, Multiplexing, and Neutralizing.
物理的形状
Purified mouse monoclonal antibody IgG1 in buffer containing PBS without preservatives.
保管および安定性
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF2786-25UL:
MABF2786-100UL:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)