由来生物
rat
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
5A1, monoclonal
分子量
calculated mol wt 60 kDa
observed mol wt ~N/A kDa
精製方法
using protein G
化学種の反応性
mouse
包装
antibody small pack of 100
テクニック
ELISA: suitable
inhibition assay: suitable
neutralization: suitable
radioimmunoassay: suitable
アイソタイプ
IgG1κ
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
-10 to -25°C
遺伝子情報
mouse ... Csf1(12977)
特異性
Clone 5A1 is a rat monoclonal antibody that detects Macrophage colony-stimulating factor 1 (CSF-1).
免疫原
Purified, serum-free, stage 4 CSF-1 from murine L cell-conditioned medium.
アプリケーション
Quality Control Testing
Evaluated by ELISA with recombinant mouse CSF-1.
ELISA Analysis: Serial dilutions of this antibody detected recombinant mouse CSF-1.
Tested Applications
Neutralizing: A representative lot neutralized the colony-stimulating activity of CSF-1.(Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
ELISA Analysis: A representative lot detected CSF-1 in ELISA applications (Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Radioimmunoassay: A representative lot detected CSF-1 in Radioimmunoassay applications (Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Inhibition: A representative lot bound and inhibited the biologic function of CSF-1.(Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by ELISA with recombinant mouse CSF-1.
ELISA Analysis: Serial dilutions of this antibody detected recombinant mouse CSF-1.
Tested Applications
Neutralizing: A representative lot neutralized the colony-stimulating activity of CSF-1.(Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
ELISA Analysis: A representative lot detected CSF-1 in ELISA applications (Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Radioimmunoassay: A representative lot detected CSF-1 in Radioimmunoassay applications (Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Inhibition: A representative lot bound and inhibited the biologic function of CSF-1.(Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
ターゲットの説明
Macrophage colony-stimulating factor 1 (UniProt: P07141; also known as CSF-1, MCSF) is encoded by the Csf1 (also known as Csfm) gene (Gene ID: 12977) in murine species. CSF-1 is a widely expressed, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 33-492), a transmembrane domain (aa 493-515), and a cytoplasmic domain (aa 516-552). Following its synthesis, it can be cleaved into a 43 kDa subunit CSF1 and a processed secreted form. CSF-1 is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells and promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Its actions are mediated by a high affinity CSF1-receptor. Clone 5A1 is shown to bind and inhibit the biological functions of CSF-1. It is reported to inhibit colony formation of tissue mononuclear phagocyte colony-forming cells as well as the committed bone marrow stem cells for both granulocytes and monocytes. The extent of colony inhibition by this clone is dependent on the tissue origin of colony-forming cells. Two isoforms of CSF-1 have been described that are produced by alternative splicing. (Ref.: Wei, S., et al. (2006). J. Leukoc. Biol. 80(6); 1445-1453; Lokeshwar, BL., and Lin, H-S. (1988). J. Immunol. 41(2); 483-488).
物理的形状
Purified rat monoclonal antibody IgG1 in PBS without azide.
再構成
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF2081-25UG:
MABF2081-100UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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