由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
15G3, monoclonal
化学種の反応性
human
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... LIN28B(389421)
詳細
Lin28 is a developmentally regulated RNA binding protein (RBP). It’s down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.
特異性
This antibody does not cross react with Lin-28A.
免疫原
Recombinant protein corresponding to human Lin28B.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
核受容体
核受容体
Anti-Lin-28B, clone 15G3 | MABE885 is an antibody against Lin-28B for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Lin28B in 10 µg of NTera2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected Lin28B in K-562 cells (Prof. N. Kim, Seoul National University).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28B in Huh7 cell lysate (Prof. N. Kim, Seoul National University).
Immunocytochemistry Analysis: A representative lot detected Lin28B in K-562 cells (Prof. N. Kim, Seoul National University).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28B in Huh7 cell lysate (Prof. N. Kim, Seoul National University).
品質
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Lin28B in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Lin28B in 10 µg of HepG2 cell lysate.
ターゲットの説明
~30 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~20 kDa
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE885:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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