由来生物
rabbit
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
RM161, monoclonal
化学種の反応性
human
テクニック
multiplexing: suitable
western blot: suitable
アイソタイプ
IgG
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
acetylation (Lys9)
遺伝子情報
human ... H3F3B(3021)
詳細
Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
特異性
This antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in Histone H3.
免疫原
A linear peptide corresponding to human Histone H3 acetylated at Lys9.
アプリケーション
This Anti-acetyl-Histone H3 (Lys9) Antibody, clone RM161 is validated for use in Western Blotting, Multiplexing for the detection of acetyl-Histone H3.
品質
Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 0.25 μg/mL of this antibody detected acetyl-Histone H3 (Lys9) in sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 0.25 μg/mL of this antibody detected acetyl-Histone H3 (Lys9) in sodium butyrate treated HeLa acid extract.
ターゲットの説明
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Format: Purified
その他情報
Concentration: Please refer to lot specific datasheet.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
does not flash
引火点(℃)
does not flash
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE645:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
William J Lu-Culligan et al.
Nature, 622(7981), 173-179 (2023-09-21)
Lysine residues in histones and other proteins can be modified by post-translational modifications that encode regulatory information1. Lysine acetylation and methylation are especially important for regulating chromatin and gene expression2-4. Pathways involving these post-translational modifications are targets for clinically approved
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