MABE2022

Anti-GINS2/PSF2 Antibody, clone 78C

• 別名: DNA replication complex GINS protein PSF2;GINS complex subunit 2
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 78C, monoclonal
• 分子量: calculated mol wt 21.43 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 包装: antibody small pack of 100 μg
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2a
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: NP_057179
• UniProtアクセッション番号: Q9Y248
• 輸送温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GINS2(51659)

詳細

詳細

DNA replication complex GINS protein PSF2 (UniProt: Q9Y248; also known as GINS complex subunit 2) is encoded by the GINS2 (also known as PSF2, CGI-122, DC5, HSPC037) gene (Gene ID: 51659) in human. GINS/PSF2 is a component of the GINS complex, which is a heterotetramer of GINS 1, 2, 3, and 4 that are assembled with 1:1:1:1 stoichiometry. Each of these four subunits is essential for proper completion of DNA replication initiation and elongation. This complex plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex binds preferentially to single-stranded DNA. At the G1/S transition, GINS is recruited to the origins of replication where it assembles with cell-division cycle protein Cdc45 and the minichromosome maintenance mutant (MCM)2-7 to form the Cdc45/Mcm2-7/GINS (CMG) complex, the presumed replicative helicase. GINS2 gene is reported to be frequently up-regulated in the cancer cells. Transfection of ETK-1 and HuH28 cells with PSF2 siRNA is shown to reduce the amount of transcript and suppress cell growth, indicating the important role of GINS2/PF2 in development of cholangiocarcinoma. (Ref.: Aparicio, T., et al. (2009). Nucl. Acids Res. 37(7); 2087-2095; Boskovic, J., et al. (2007). EMBO Rep. 8(7); 678-684; Obama, K., et al. (2005). Oncol. Rep. 14(3):701-706).

特異性

Clone 78C is a mouse monoclonal antibody that detects human DNA replication complex GINS protein PSF2 (GINS2/PSF2).

免疫原

Recombinant human GINS2/PSF2 expressed in E. coli.

アプリケーション

Quality Control Testing

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected GINS2/PSF2 in A431 cells.

Tested Applications

Western Blotting Analysis: A representative lot detected GINS2/PSF2 in Western Blotting application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Immunocytochemistry Analysis: A representative lot detected GINS2/PSF2 in Immunocytochemistry application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Immunoprecipitation Analysis: A representative lot immunoprecipitated GINS2/PSF2 in Immunoprecipitation application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-GINS2/PSF2, clone 78C, Cat. No. MABE2022, is a mouse monoclonal antibody that detects GINS/PSF2 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable