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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
4B9.1, monoclonal
化学種の反応性
human
包装
antibody small pack of 25 μg
テクニック
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG2bκ
NCBIアクセッション番号
UniProtアクセッション番号
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CCAR1(55749)
詳細
Cell division cycle and apoptosis regulator protein 1 (UniProt: Q8IX12; also known as Cell cycle and apoptosis regulatory protein 1, CARP-1, Death inducer with SAP domain, CCAR1) is encoded by the CCAR1 (also known as CARP1, DIS) gene (Gene ID: 55749) in human. CCAR1 is a cytoplasmic protein that localized to the perinuclear region. It is expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Its expression is shown to be regulated by growth factors. Two isoforms of CCAR1 have been reported that are generated by alternative splicing. CCAR1 associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. CCAR1 is recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. It also functions as a p53 coactivator and may play a role in transcriptional regulation. It is reported to be involved in apoptosis signaling in the presence of the reinoid CD437. In association with CALCOCO1, it enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells. CCAR1 can act as a coactivator and corepressor of AR-mediated transcription and contribute to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites.
特異性
Clone 4B9.1 detects Cell division cycle and apoptosis regulator protein 1 (CCAR1) in human cells. It targets an epitope witihin 173 amino acids from the N-terminal half.
免疫原
GST/His-tagged recombinant fragment corresponding to 173 amino acids from the N-terminal half of human Cell division cycle and apoptosis regulator protein 1 (CCAR1).
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Anti-CCAR1, clone 4B9.1, Cat. No. MABE1671, is a highly specific mouse monoclonal antibody that targets Cell division cycle and apoptosis regulator protein 1 and has been tested in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected CCAR1 in HeLa, A431, and HUVEC cells.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected CCAR1 in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected CCAR1 in 10 µg of HepG2 cell lysate.
品質
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CCAR1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CCAR1 in 10 µg of HeLa cell lysate.
ターゲットの説明
~135 kDa observed; 132.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE1671:
MABE1671-25UG:
試験成績書(COA)
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