MABE1002

Anti-DIS3-like Exonuclease 2 Antibody, clone 9H2.1

clone 9H2.1, from mouse

• 別名: hDIS3L2, DIS3-like Exonuclease 2, DIS3L2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 9H2.1, monoclonal
• 交差性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_689596
• UniProtアクセッション番号: Q8IYB7
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DIS3L2(129563)

詳細

詳細

DIS3-like exonuclease 2 (UniProt Q8IYB7; also known as DIS3 mitotic control homolog-like 2, DIS3L2, Family with sequence similarity 6 member A, hDIS3L2) is encoded by the DIS3L2 (also known as FAM6A, PRLMNS) gene (Gene ID 129563) in human. Cytoplasmic mRNAs are regulated by degradations to ensure a fast and efficient control of the transcriptome at the post‐transcriptional level. The degradation process is initiated by deadenylation, followed by decapping, after which mRNA is accessible to digestion by the Xrn1 exonuclease in the 5′‐3′ direction or by the exosome complex in the 3′‐5′ direction. In yeast, the cytoplasmic exosome consists of nine inactive subunits (core ring structure) plus an active ribonuclease Rrp44/Dis3. Three Dis3 isoforms exist in human, DIS3, DIS3L, and DIS3L2. DIS3 is nuclear, and DIS3L and DIS3L2 are cytoplasmic. In addtion to mRNAs, DIS3L2 is also reported to mediate the degradtion of 3′ uridylated let-7 precursor (pre-let-7) miRNA. Lin28 is a pluripotency factor expressed in stem cells and cancer cells, where it helps maintain the cells in an undifferentiated and proliferative state by preventing miRNA let-7 maturation. Lin28 binds to pre-let-7 and recruits 3′ terminal uridylyl transferase, which in turn marks pre-let-7 via 3′ oligouridylation for degradation by DIS3L2. Cellular DIS3L2 depletion causes mitotic abnormalities due to the deregulation of the expression of mitotic control proteins and DIS3L2 gene mutations are linked to the congenital overgrowth disease Perlman syndrome.

特異性

Expected to react with isoforms 1-4. Reactivity toward isoform 5 has not been determined.

免疫原

GST-tagged recombinant protein corresponding to the Internal (N-terminal half) of human DIS3-like Exonuclease 2.

アプリケーション

Anti-DIS3-like Exonuclease 2 Antibody, clone 9H2.1 is an antibody against DIS3-like Exonuclease 2 Antibody for use in Western Blotting.

品質

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DIS3-like Exonuclease 2 in 10 µg of HepG2 cell lysate.

ターゲットの説明

~100 kDa observed. 99.3 kDa (Isoform 1), 69.0 kDa (Isoform 2), 67.3 kDa (Isoform 3), 27.5 kDa (Isoform 4), 40.5 kDa (Isoform 5) calculated.

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable