MABC112

Anti-RASSF1 Antibody, clone 5C9.1

clone 5C9.1, from mouse

• 別名: Ras association domain-containing protein 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 5C9.1, monoclonal
• 化学種の反応性: human
• テクニック: immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_009113
• UniProtアクセッション番号: Q9NS23
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RASSF1(11186)

詳細

詳細

RASSF1, also known as Ras association domain-containing protein 1, and encoded by the gene RASSF1/RDA32, is an important cell cycle and cell proliferation regulator protein. RASSF1 acts as a tumor suppressor and operates within the death receptor dependent apoptotic pathways. Additionally, RASSF1 inhibits proliferation at the G1/S boundary by regulating the accumulation of cyclin D1 protein. RASSF1 is localized to the cytoplasm and cytoskeleton depending upon cellular environment. RASSF1 localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. The protein also co-localizes with MDM2 in the nucleus. RASSF1 is expressed in multiple isoforms but in generally it is ubiquitously expressed but down regulated in cancer and tumor cells.

免疫原

GST-tagged recombinant protein corresponding to human RASSF1.

アプリケーション

Detect RASSF1 using this Anti-RASSF1 Antibody, clone 5C9.1 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:50-500 dilution from a representative lot detected RASSF1 in human kidney and human prostate cancer tissue.

品質

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected RASSF1 in 10 µg of human brain tissue lysate.

ターゲットの説明

~39 kDa observed

関連事項

Replaces: MABE526

物理的形状

Format: Purified

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable