MAB353A4

Anti-Nestin Antibody, clone rat-401, Alexa Fluor^™ 488 conjugate

clone rat-401, from mouse, ALEXA FLUOR^™ 488

• 別名: rat401, NES, Nbla00170, FLJ21841
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 488
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: rat-401, monoclonal
• 化学種の反応性: rat, mouse
• 以下との反応禁止: human
• テクニック: immunocytochemistry: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NP_037119
• UniProtアクセッション番号: P21263
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

詳細

詳細

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

特異性

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

免疫原

Embryonic rat spinal cord

アプリケーション

Research Category
幹細胞研究

ニューロサイエンス

Research Sub Category
ニューロフィラメント及び神経細胞代謝

神経細胞及びグリアマーカー

Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).

品質

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

ターゲットの説明

The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

物理的形状

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

保管および安定性

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

アナリシスノート

Control
Mouse cortical neural stem cells (SCR029)

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable