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由来生物
mouse
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
monoclonal
化学種の反応性
human, mouse
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
western blot: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... FLT1(2321)
特異性
Recognizes Human Flt-1 (fms-like tyrosine kinase). Flt-1 (human 1338 aa, rat 1336 aa, and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt-1 has also been described as VEGFR1 and Flk-2. It is type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor cells (Shibuya et al., 1990; Matsushime et al., 1987; Yamane et al., 1994; Finnerty et al., 1993; Choi et al., 1994; Plouet et al., 1989; Simon et al.,
1998; Sait et al., 1995; deVries et al., 1992).
SPECIES REACTIVITIES:
Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.
1998; Sait et al., 1995; deVries et al., 1992).
SPECIES REACTIVITIES:
Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.
免疫原
Human recombinant Flt-1 (extracellular domain). Actual epitope recognized by this clone is between 1-250 aa of human Flt-1.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
成長因子及び受容体
成長因子及び受容体
Anti-VEGF Receptor-1 Antibody is an antibody against VEGF Receptor-1 for use in ELISA & WB.
Western blot: 0.5-2.0 μg/mL. MW = 180 kDa.
Flow cytometry: Dilute the antibody to 25 μg/mL and use 10-20 μL antibody for 1-2 x 10E5 cells in a total volume of 200-250 μL. Visualize with appropriate secondary antibodies.
ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.
Optimal working dilutions must be determined by end user.
Flow cytometry: Dilute the antibody to 25 μg/mL and use 10-20 μL antibody for 1-2 x 10E5 cells in a total volume of 200-250 μL. Visualize with appropriate secondary antibodies.
ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.
Optimal working dilutions must be determined by end user.
物理的形状
Format: Purified
Purified from Ascites. Liquid in PBS and 5% Trehalose.
保管および安定性
Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze and thaw.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB1664:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Motoyuki Otsuka et al.
The Journal of clinical investigation, 118(5), 1944-1954 (2008-04-10)
The microRNA (miRNA) processing enzyme Dicer1 is required for zygotic and embryonic development, but the early embryonic lethality of Dicer1 null alleles in mice has limited our ability to address the role of Dicer1 in normal mouse growth and development.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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