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詳細
The rigidity of the cell′s substrate (such as the extracellular matrix) can have a profound effect on cell function including morphology, differentiation and gene expression. Young′s elastic modulus is a mechanical property that measures the stiffness of a solid material. It defines the relationship between stress and strain in a material and is measured in kPa.
CytoSoft® plates provide a tool to culture cells on substrates with defined rigidities covering a broad physiological range (0.2 kPa- 64 kPa). On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The surfaces of the gels are functionalized to form covalent bonds with amines on proteins. The gel readily binds to purified ECM proteins, such as PureCol type I collagen (5006), prior to cell addition.
CytoSoft® Imaging plates are designed for high-resolution imaging where low autofluorescence and exceptional optical clarity are required. These plates accommodate both live cell imaging and common fixation techniques such as immunostaining. Plate consists of a 175 μm thin polycarbonate film-bottom plate bonded with a black polystyrene frame and includes a lid. The plates are sterilized using ozone and provided with 1 plate per package.
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. The bottom of the plate can be detached by excessive mechanical force such as centrifugation or direct contact with liquid handling tools (tips, pipettes, etc.).
1. Remove the CytoSoft® product from the protective sleeve in a sterile hood.
2. Prepare extracellular matrix material by neutralizing in amine-free buffer pH 7.4 to 7.9 (such as 1X DPBS). We do not recommend using gelatin as your ECM protein. Note: Pre-warm the coating solution to approximately room temperature before use.
3. Dilute as needed, and dispense 1 mL of solution into each well to coat the surface. Note: Recommended dilution for PureCol Type I collagen is 1:30 (~100 μg/ml). Note: The hydrophobic surface requires larger volumes to cover the surface than do conventional plastic dishes
4. Incubate ECM coated CytoSoft® at room temperature, covered for 1 hour.
5. After incubation, aspirate any remaining material and rinse coated surfaces immediately two times with culture medium or PBS. Leave about 2.5 mL of medium per well to keep surface covered.Note: Do not allow the CytoSoft® surface to become dry once the surface has been wetted.
6. Coated surfaces are ready for use. Standard harvesting procedures used for removing cells from cultureware can be employed for harvesting cells from the CytoSoft® product including use of trypsin, Accutase and non-enzymatic cell detachment solutions.
CytoSoft® is a registered trademark of Advanced BioMatrix, Inc.
CytoSoft® plates provide a tool to culture cells on substrates with defined rigidities covering a broad physiological range (0.2 kPa- 64 kPa). On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The surfaces of the gels are functionalized to form covalent bonds with amines on proteins. The gel readily binds to purified ECM proteins, such as PureCol type I collagen (5006), prior to cell addition.
CytoSoft® Imaging plates are designed for high-resolution imaging where low autofluorescence and exceptional optical clarity are required. These plates accommodate both live cell imaging and common fixation techniques such as immunostaining. Plate consists of a 175 μm thin polycarbonate film-bottom plate bonded with a black polystyrene frame and includes a lid. The plates are sterilized using ozone and provided with 1 plate per package.
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. The bottom of the plate can be detached by excessive mechanical force such as centrifugation or direct contact with liquid handling tools (tips, pipettes, etc.).
1. Remove the CytoSoft® product from the protective sleeve in a sterile hood.
2. Prepare extracellular matrix material by neutralizing in amine-free buffer pH 7.4 to 7.9 (such as 1X DPBS). We do not recommend using gelatin as your ECM protein. Note: Pre-warm the coating solution to approximately room temperature before use.
3. Dilute as needed, and dispense 1 mL of solution into each well to coat the surface. Note: Recommended dilution for PureCol Type I collagen is 1:30 (~100 μg/ml). Note: The hydrophobic surface requires larger volumes to cover the surface than do conventional plastic dishes
4. Incubate ECM coated CytoSoft® at room temperature, covered for 1 hour.
5. After incubation, aspirate any remaining material and rinse coated surfaces immediately two times with culture medium or PBS. Leave about 2.5 mL of medium per well to keep surface covered.Note: Do not allow the CytoSoft® surface to become dry once the surface has been wetted.
6. Coated surfaces are ready for use. Standard harvesting procedures used for removing cells from cultureware can be employed for harvesting cells from the CytoSoft® product including use of trypsin, Accutase and non-enzymatic cell detachment solutions.
CytoSoft® is a registered trademark of Advanced BioMatrix, Inc.
保管および安定性
Store CytoSoft Imaging plates at room temperature.
法的情報
CytoSoft is a registered trademark of Advanced BioMatrix, Inc.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
13 - Non Combustible Solids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
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名称等を通知すべき危険物及び有害物
Jan Code
CC315:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
CytoSoft® modulus plates analyze substrate stiffness effects on cell behavior across a physiological range for mechanobiology research.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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