ABS218

Anti-phospho-DBC1 (Thr454) Antibody

from rabbit, purified by affinity chromatography

• 別名: DBIRD complex subunit KIAA1967, Deleted in breast cancer gene 1 protein, DBC-1, DBC.1, p30 DBC
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• テクニック: inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_066997
• UniProtアクセッション番号: Q8N163
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pThr454)
• 遺伝子情報: human ... BRINP1(1620)

詳細

詳細

Deleted in breast cancer gene 1 protein (DBC1) is a large, nuclear protein with multiple functions in cell survival that binds directly to the estrogen receptor α (ERα) hormone-binding domain in a strict ligand-independent manner. DBC1 promotes p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity. It is ubiquitously expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.

特異性

This antibody recognizes DBC1 phosphorylated at Thr454.

免疫原

Epitope: Phosphorylated Thr454

KLH-conjugated linear peptide corresponding to human DBC-1 phosphorylated at Thr454.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
発生シグナル伝達

Anti-phospho-DBC1 (Thr454) Antibody is an antibody against phospho-DBC1 (Thr454) for use in Western Blotting, Peptide Inhibition Assay.

Peptide Inhibition Assay: A 1:500 dilution from a representative lot blocked DBC-1 phosphorylated at Thr454 in untreated and etoposide treated Jurkat cell lysates.

品質

Evaluated by Western Blot in untreated and etoposide treated Jurkat cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DBC-1 phosphorylated at Thr454 in 10 µg of etoposide treated Jurkat cell lysates.

ターゲットの説明

~130 kDa observed. Uniprot states the calculated molecular weight is 103 kDa but has been shown at ~130kDa (Satoh, M., et al. (1995). Brain Res Dev Brain Res. 87(2):111-119).

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Untreated and etoposide treated Jurkat cell lysates

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable