由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, rat
テクニック
immunohistochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... GSTP1(2950)
詳細
Glutathione S-transferase P (EC 2.5.1.18; UniProt P04906; also known as Chain 7, GST 7-7, GST class-pi) is encoded by the Gstp1 gene (Gene ID 24426) in rat species. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist as either homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (Mr 24.5 kDa), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The Y designation refers to the Y fraction (as apposed to the X and Z fractions) they were found in during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes. namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma, theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTP exists as a homodimer composed of two Yf subunits.
特異性
Reacts with human and rat glutathione S-transferase P dimer (Yf/Yf) and monomer (Yf).
免疫原
Purified rat liver glutathione S-transferase P.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
酸化ストレス
酸化ストレス
Anti-Gstp1 Antibody is an antibody against Gstp1 for use in Western Blotting, Immunohistochemistry.
Western Blotting Analysis: A representative lot detected glutathione S-transferase P in rat liver and human stomach tissue homogenates (Cobbe,S.C., et al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47).
Western Blotting Analysis: A representative lot detected upregulated glutathione S-transferase P (GSTP1) levels in liver homogenates from rats on diets supplemented with either one of six (EQ, CMRN, BHA, DEM, t-SO, or β-NF), but not with four other phytochemicals (OPZ, I3C, BITC, or PB) tested (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
Immunohistochemistry Analysis: A representative lot detected much reduced glutathione S-transferase P (GSTP1) immunoreactivity in paraffin-embedded Barrett′s oesophagus tissues when compared to normal human oesophagus tissue sections (Cobbe,S.C., et al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47).
Immunohistochemistry Analysis: A representative lot detected enhanced Glutathione S-transferase P immunoreactivity in the centrilobular part of liver from rats received dietary coumarin (CMRN) using acetone-fixed, paraffin-embedded tissue sections (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
Western Blotting Analysis: A representative lot detected upregulated glutathione S-transferase P (GSTP1) levels in liver homogenates from rats on diets supplemented with either one of six (EQ, CMRN, BHA, DEM, t-SO, or β-NF), but not with four other phytochemicals (OPZ, I3C, BITC, or PB) tested (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
Immunohistochemistry Analysis: A representative lot detected much reduced glutathione S-transferase P (GSTP1) immunoreactivity in paraffin-embedded Barrett′s oesophagus tissues when compared to normal human oesophagus tissue sections (Cobbe,S.C., et al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47).
Immunohistochemistry Analysis: A representative lot detected enhanced Glutathione S-transferase P immunoreactivity in the centrilobular part of liver from rats received dietary coumarin (CMRN) using acetone-fixed, paraffin-embedded tissue sections (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
品質
Evaluated by Western Blotting in human lung tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GSTP1 Yf monomer in 10 µg of human lung tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GSTP1 Yf monomer in 10 µg of human lung tissue lysate.
ターゲットの説明
~25 kDa observed (Yf monomer).
物理的形状
Unpurified
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABS1650:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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