由来生物
rabbit
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
分子量
calculated mol wt 17.74 kDa
observed mol wt ~20 kDa
精製方法
affinity chromatography
化学種の反応性
human
包装
antibody small pack of 100 μg
テクニック
immunocytochemistry: suitable
western blot: suitable
アイソタイプ
IgG
エピトープ配列
Internal
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... FUS(2521)
詳細
AltFus (UniProt: A0A823ADP9; also known as Alt-Fus) is encoded by the FUS gene (Gene ID: 2521) in human. FUS is a nuclear RNA-binding protein with a C-terminal nuclear localization signal. It is involved in RNA processing, DNA repair, and cell proliferation. FUS is a dual coding gene that also codes for altFUS, a highly conserved, 170 amino acid protein that is endogenously expressed in many cell lines and tissues, including the motor cortex and motor neurons. Higher expression of FUS as well as altFUS contribute to neuronal toxicity. AltFUS is embedded within the FUS gene coding sequence. It is mainly a mitochondrial protein, but a weak cytoplasmic signal is also detected. Cells overexpressing altFUS display altered mitochondrial network, with a higher number of fragmented mitochondria. It can also lead to a loss in mitochondrial potential and accumulation of cytoplasmic aggregates. AltFUS is reported to inhibit autophagy and drive the accumulation of FUS- and TDP43-positive cytoplasmic aggregates. Suppression of its expression in a Drosophila model of FUS-related toxicity is shown to protect against neurodegeneration. (Ref.: Brunet, MA., et al. (3021). EMBO Rep. 22; e50640).
特異性
This rabbit polyclonal antibody specifically detects a mitochondrial specific 170 amino acid protein
免疫原
Linear peptides corresponding to 19 amino acids from the N-terminal region and 15 amino acids from the internal region of mitochondrial specific human altFUS.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in Human brain tissue lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected altFUS in Human brain tissue lysates.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected altFUS in Immunocytochemistry applications (Brunet, M.A., et. al. (2021). EMBO Rep. 22(1); e50640).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human brain tissue lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected altFUS in Human brain tissue lysates.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected altFUS in Immunocytochemistry applications (Brunet, M.A., et. al. (2021). EMBO Rep. 22(1); e50640).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
物理的形状
Purified rabbit polyclonal antibody in PBS with 0.01% sodium azide.
再構成
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommended storage: +2°C to +8°C.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABN2247-100UG:
ABN2247-25UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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