ABE552

Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody

from rabbit, purified by affinity chromatography

• 別名: Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: monkey (based on 100% sequence homology), sheep (based on 100% sequence homology), ox (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_001073901
• UniProtアクセッション番号: Q9C0B1
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FTO(79068)

詳細

詳細

Alpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.

免疫原

KLH-conjugated linear peptide corresponding to human Alpha-ketoglutarate-dependent dioxygenase FTO.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Immunoprecipitation Analysis: 10 μg from a representative lot immunoprecipitated Alpha-ketoglutarate-dependent dioxygenase FTO from 500 μg of U2OS nuclear extract.

This Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody is validated for use in western blotting & IP for the detection of Alpha-ketoglutarate-dependent dioxygenase FTO.

品質

Evaluated by Western Blotting in U2OS nuclear extract.

Western Blotting Analysis: 1 μg/mL of this antibody detected Alpha-ketoglutarate-dependent dioxygenase FTO in 10 μg of U2OS nuclear extract.

ターゲットの説明

~58 kDa observed

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable