由来生物
sheep
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
human, mouse
テクニック
ChIP: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SHMT1(6470)
詳細
Serine hydroxymethyltransferase (SHMT) is also known as glycine hydroxymethyltransferase or serine methylase, and is an enzyme that catalyzes the reversible conversion of serine and glycine. SHMT is thought to be involved in the epigenetic DNA methylation mechanism and has shown increased activity during cell proliferation and the S phase of the cell cycle. Evidence indicates that SHMT repression is a critical step in the Retinoic Acid-induced cell growth arrest and differentiation pathway.
免疫原
KLH-conjugated linear peptide corresponding to mouse SHMT1.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
エピジェネティクス
エピジェネティクス
Anti-SHMT1 Antibody is a highly specific sheep polyclonal antibody, that targets Serine hydroxymethyltransferase & has been tested in western blotting.
Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of mouse liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).
Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected SHMT1 when coprecipitated with PCNA in pBABE-Puro-SV50-LT transfected HeLa nuclear extracts. MacFarlane A.J., et al. (2011). J Biol Chem. 286(51):44015-22
Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected SHMT1 when coprecipitated with PCNA in pBABE-Puro-SV50-LT transfected HeLa nuclear extracts. MacFarlane A.J., et al. (2011). J Biol Chem. 286(51):44015-22
品質
Evaluated by Western Blot in human liver tissue lysate.
Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of human liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).
Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of human liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).
ターゲットの説明
~53 kDa observed. An uncharacterized band may be observed at ~40 kDa in some tissue lysates.
物理的形状
Affinity purified
Purified sheep polyclonal in buffer containing 0.5M HEPES with 0.05% sodium azide and 50% glycerol.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
アナリシスノート
Control
Human liver tissue lysate
Human liver tissue lysate
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 製品選択ツール.
保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE416:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）