ABE2927

Anti-Histone H3 K4M mutant

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 分子量: calculated mol wt 15.33 kDa; observed mol wt ~18 kDa
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 包装: antibody small pack of 100 μL
• テクニック: immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: N-terminal
• タンパク質IDアクセッション番号: NP_001365972
• UniProtアクセッション番号: P84243
• 輸送温度: 2-8°C
• ターゲットの翻訳後修飾: mutation (Lys4Met)

詳細

詳細

Histone H3.3 (UniProt: P84243; also known as H3.3) is encoded by the H3F3A (also known as H3.3A, H3F3, PP781, H3F3B, H3.3B) gene (Gene ID: 3020; 3021) in human. Histone H3.3 is a highly conserved variant form of Histone H3, which replaces conventional H3 in a wide range of nucleosomes in active genes. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H3.1 serves as the canonical histone, which is incorporated during DNA replication, whereas H3.3 acts as the replacement histone that can be incorporated outside of S-phase during chromatin-disrupting processes like transcription. Histone H 3.3 constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. It is associated with actively expressed genes in both animals and plants. It is predominantly enriched near transcription end sites (TES) of genes and positively associated with transcription. Histone H3 contains a main globular domain and a long N-terminal tail and is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Mutations in Histone H3.3 have been implicated in a high proportion of malignant pediatric brain cancers. K4M mutation is reported to inhibit H3K4 methylation and prevent enhancer activation in adipogenesis by destabilizing MLL4/MLL4 proteins, but does not affect MLL1, MLL2, SETA1, or SET1B. Expression of K4M in lineage-specific precursor cells is shown to deplete H3K4 methylation and impair adipose tissue and muscle development. (Ref.: Jang, Y., et al. (2019). Nucleic Acids Res. 47(2); 607-620; Tagami, H., et al. (2004). Cell, 116(1); 51-61).

特異性

This rabbit polyclonal antibody detected K4M and K4I mutant forms of Histone H3.

免疫原

KLH-conjugated linear peptide corresponding to the first 9 amino acids (without first methionine) from the N-terminal region of Histone H3 with K4M mutation.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HEK293T cells transfected with K4M mutant.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histone H3.3 in HEK293T cells transfected with K4M mutation.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Histone H3.3 in HEK293T cells transfected with K4M mutant (Courtesy of Dave Allis Lab @ Rockefeller University).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Histone H3.3 in HEK293T cells transfected with K4M mutant (Courtesy of Dave Allis Lab @ Rockefeller University).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Histone H3 K4M mutant, Cat. No. ABE2927, is a rabbit polyclonal antibody that detects Histone H3 with K4M mutation and is tested for use in Immunofluorescence and Western Blotting.

物理的形状

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable