由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse, Xenopus
テクニック
ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MCM9(254394)
関連するカテゴリー
詳細
DNA helicase MCM9 (EC 3.6.4.12; UniProt Q9NXL9; also known as hMCM9, Mini-chromosome maintenance deficient domain-containing protein 1) is encoded by the MCM9 (also known as C6orf61, MCMDC1, ODG4) gene (Gene ID 254394) in human. Mammalian minichromosome maintenance (MCM) protein homologues MCM8 and MCM9 play important roles in DNA replication elongation and prereplication complex (pre-RC) formation. MCM9 is found physically interacted with MCM8 and the interaction is essential for MCM9 cellular stability. MCM8-MCM9 complex is required for homologous recombination- (HR-) mediated repair of double-strand breaks (DSBs). In addition, the ATPase motifs of MCM8 and MCM9 (a.a. 523-530) are needed for MRN (MRE11-RAD50-NBS1) complex recruitment to DSBs sites. Cancer cells with MCM9 homozygous deletion display enhanced reduction in cell survival following exposure to interstrand-crosslinking (ICL) agents, such as cisplatin, due to impaired HR efficiency.
特異性
This rabbit polyclonal antiserum detected DNA damage-induced MCM9 nuclear foci formation in U2OS and wild-type, but not Mcm9-null MEFs (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644). The C-terminal end epitope region is present in all four human MCM9 spliced isoforms and the first two mouse MCM9 isoforms reported by UniProt (Q9NXL9 and Q2KHI9).
免疫原
Epitope: C-terminus
Recombinant human MCM9 C-terminal fragment.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Anti-MCM9, Cat. No. ABE2603, is a highly specific rabbit polyclonal antibody that targets DNA helicase MCM9 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected a time-dependent MCM9 recruitment to the integrated I-scel cleavage site in a RAD51-independent manner in HeLa DR13-9 cells following I-scel transfection (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected a time-dependent MCM9 recruitment to the intrastrand cross-link (ICL) site of the pICL plasmid in xenopus egg extract followng induction of replication-dependent repair (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
Immunocytochemistry Analysis: A representative lot detected DNA damage-induced MCM9 nuclear foci formation co-colocalized with RPA immunoreactivity by fluorescent immunocytochemistry staining of 0.5% Triton X-100-permeabilized, 4% paraformaldehyde-fixed human U2OS and mouse embryonic fibroblasts (MEFs) following cisplatin treatment. No target staining was seen in Mcm9-null MEFs (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
Immunoprecipitation Analysis: A representative lot co-immunoprecipiated MRN (MRE11-RAD50-NBS1) complex with MCM9 from U2OS and HEK293T cells (Lee, K.Y., et al. (2015). Nat. Commun. 6:7744).
Immunoprecipitation Analysis: A representative lot co-immunoprecipiated MCM8 and RAD51 with MCM9 from U2OS and HEK293T nuclear extracts. The antiserum also immunodepleted MCM9 in xenopus egg extract (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
Western Blotting Analysis: A representative lot detected MCM9 downreguation in prostate cancer cells and NCI-H1299, but not NCI-H2291, non-small cell lung cancer (NSCLC) cells fig1, 2, S1 (Lee, K.Y., et al. (2015). Nat. Commun. 6:7744).
Western Blotting Analysis: Representative lots detected MCM9 in U2OS cells. Transfecting cells with shRNA targeting either MCM8 or MCM9 downregulated cellular MCM9 level (Lee, K.Y., et al. (2015). Nat. Commun. 6:7744; Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected a time-dependent MCM9 recruitment to the intrastrand cross-link (ICL) site of the pICL plasmid in xenopus egg extract followng induction of replication-dependent repair (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
Immunocytochemistry Analysis: A representative lot detected DNA damage-induced MCM9 nuclear foci formation co-colocalized with RPA immunoreactivity by fluorescent immunocytochemistry staining of 0.5% Triton X-100-permeabilized, 4% paraformaldehyde-fixed human U2OS and mouse embryonic fibroblasts (MEFs) following cisplatin treatment. No target staining was seen in Mcm9-null MEFs (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
Immunoprecipitation Analysis: A representative lot co-immunoprecipiated MRN (MRE11-RAD50-NBS1) complex with MCM9 from U2OS and HEK293T cells (Lee, K.Y., et al. (2015). Nat. Commun. 6:7744).
Immunoprecipitation Analysis: A representative lot co-immunoprecipiated MCM8 and RAD51 with MCM9 from U2OS and HEK293T nuclear extracts. The antiserum also immunodepleted MCM9 in xenopus egg extract (Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
Western Blotting Analysis: A representative lot detected MCM9 downreguation in prostate cancer cells and NCI-H1299, but not NCI-H2291, non-small cell lung cancer (NSCLC) cells fig1, 2, S1 (Lee, K.Y., et al. (2015). Nat. Commun. 6:7744).
Western Blotting Analysis: Representative lots detected MCM9 in U2OS cells. Transfecting cells with shRNA targeting either MCM8 or MCM9 downregulated cellular MCM9 level (Lee, K.Y., et al. (2015). Nat. Commun. 6:7744; Park, J., et al. (2013). Mol. Cell. Biol. 33(8):1632-1644).
品質
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this polyclonal antiserum detected MCM9 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this polyclonal antiserum detected MCM9 in 10 µg of HEK293 cell lysate.
ターゲットの説明
~130 kDa observed. 127.3/72.74/43.98/84.44 kDa (human isoform 1/2/3/4), 125.8/142.4 kDa (mouse isoform 1/2), 126.6 kDa (xenopus) calculated. Uncharacterized band(s) may appear in some lysates.
物理的形状
Unpurified
Rabbit polyclonal antibody serum with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE2603:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ananya Acharya et al.
Nature communications, 15(1), 3584-3584 (2024-04-28)
HROB promotes the MCM8-9 helicase in DNA damage response. To understand how HROB activates MCM8-9, we defined their interaction interface. We showed that HROB makes important yet transient contacts with both MCM8 and MCM9, and binds the MCM8-9 heterodimer with
Jen-Wei Huang et al.
Nature communications, 11(1), 2948-2948 (2020-06-13)
Homologous recombination (HR) mediates the error-free repair of DNA double-strand breaks to maintain genomic stability. Here we characterize C17orf53/MCM8IP, an OB-fold containing protein that binds ssDNA, as a DNA repair factor involved in HR. MCM8IP-deficient cells exhibit HR defects, especially
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）