ABE1337

Anti-SETD6

from rabbit, purified by affinity chromatography

• 別名: N-lysine methyltransferase SETD6, SET domain-containing protein 6
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 包装: antibody small pack of 25 μg
• テクニック: immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_001153777.1
• UniProtアクセッション番号: Q8TBK2
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SETD6(79918)

詳細

詳細

N-lysine methyltransferase SETD6 (UniProt: Q8TBK2; also known as SET domain-containing protein 6) is encoded by the SETD6 gene (Gene ID: 79918) in human. N-lysine methyltransferase SETD6 is a SET domain protein that specifically monomethylates lysine 310 of NF-kappa B-p65 that leads to down-regulation of NF-kappa B transcription factor activity. It can also monomethylate lysine 8 of H2AZ (H2AZK8me1). SETD6 is required for the maintenance of embryonic stem cell self-renewal. Primary urothelial cells and normal bladder tissue have almost undetectable SETD6 levels that are shown to increase in transformed urothelial cells and is further increased in bladder cancer cells and tissues. Overexpression of SETD6 in transformed urothelial cells increases their survival and colony formation. SETD6 is shown to interact with the oxidative stress sensor protein DJ1, which is required for Nrf2-dependent transcription of antioxidant target genes. Under basal conditions, SETD6 and DJ1 are associated at chromatin and SETD6 inhibits DJ1 activity, which leads to the repression of Nrf2-dependent transcription. However, under conditions of oxidative stress SETD6 mRNA and protein levels decline that leads to elevation in Nrf2 expression and diminish interaction between SETD6 and DJ1. Two isoforms of SETD6 have been described that are produced by alternative splicing. (Ref.: Mukherjee, N., et al. (2017). Oncotarget. 8(9); 15114-15125; Chen, A., et al. (2016). Biochim. Biophys. Acta. 1859(2):420-427).

特異性

This rabbit polyclonal antibody detects N-lysine methyltransferase SETD6 in human cells. it targets an epitope within 18 amino acids from the N-terminal region.

免疫原

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human N-lysine methyltransferase SETD6, isoform 1.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-SETD6, Cat. No. ABE1337, is a highly specific rabbit polyclonal antibody that targets N-lysine methyltransferase SETD6 and has been tested for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SETD6 in HEK293 cells.

品質

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SETD6 in human kidney tissue lysate.

ターゲットの説明

~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable