ABE1068

Anti-Lin-28B Antibody

from rabbit, purified by affinity chromatography

• 別名: Protein lin-28 homolog B, Lin-28B
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: bovine (based on 100% sequence homology), porcine (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_001004317
• UniProtアクセッション番号: Q6ZN17
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LIN28B(389421)

詳細

詳細

Lin28 is a developmentally regulated RNA binding protein (RBP). It’s down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

特異性

Other homologies: mouse, rat 83%.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human Lin-28B.

アプリケーション

Anti-Lin-28B is an antibody against Lin-28B for use in WB, IH.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Lin-28B in 10 µg of human testis tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Lin-28B in human testis and human liver cancer sections tissue.

品質

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lin-28B in 10 µg of HepG2 cell lysate.

ターゲットの説明

~35/23 kDa observed

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable