ABC212

Anti-phospho ULK1 Antibody (Thr574)

from rabbit, purified by affinity chromatography

• 別名: Serine/threonine-protein kinase ULK1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
• テクニック: inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_033495
• UniProtアクセッション番号: O70405
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pThr574)
• 遺伝子情報: human ... ULK1(8408)

詳細

詳細

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

免疫原

KLH-conjugated linear peptide corresponding to mouse ULK1 phosphorylated at Thr574.

アプリケーション

Detect Autophagy-related protein;ATG1 using this rabbit polyclonal antibody, Anti-phospho ULK1 Antibody (Thr574) validated for use in western blotting & Peptide Inhibition Assay.

Western Blotting/Peptide Inhibition Analysis: 0.5 µg/mL from a representative detected phospho ULK1 in WB/PIA.

品質

Evaluated by Western Blotting in untreated and Calyculin A with okadaic acid treated Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho ULK1 (Thr574) in 10 µg of Jurkat cell lysate.

ターゲットの説明

~120 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable