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The pTriEx<TMSYMBOL></TMSYMBOL>-6 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, pTriEx-6 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector<TMSYMBOL></TMSYMBOL> System or BacMagic<TMSYMBOL></TMSYMBOL> Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. For expression in E. coli, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. The pTriEx-6 vector contains an N-terminal 8 aa Strep•Tag<TMSYMBOL></TMSYMBOL> II coding sequence followed by a HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and His•Tag<TMSYMBOL></TMSYMBOL> coding sequence with 10 histidines. pTriEx-6 is also available as a 3C/LIC Vector Kit.
This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the ′EMD Product′) requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the ′EMD Product′) requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
警告
Toxicity: Standard Handling (A)
法的情報
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
71559-20UG:
71559-MG:
71559-3:
試験成績書（COA）
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