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詳細
Research area: Cancer
Recombinant ubiquitin fused at the N-terminus to GST and expressed in E. coli. Useful for glutathione affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using GST antibodies. Facilitates the visualization of polyUb chains due to larger ladder intervals.
Recombinant ubiquitin fused at the N-terminus to GST and expressed in E. coli. Useful for glutathione affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using GST antibodies. Facilitates the visualization of polyUb chains due to larger ladder intervals.
アプリケーション
Ubiquitin, GST-Fusion, Recombinant, E. coli has been used for in vivo and in vitro ubiquitination assays, to study the role of FBXL2-Grp94-EGFR axis in non-small cell lung cancer (NSCLC) development and the role of F-box protein 3 (FBXO3) in promotion of breast cancer metastasis.
生物化学的／生理学的作用
The ubiquitin (Ub) system plays a crucial role in protein homeostasis by maintaining protein balance in pathways related to DNA repair, cell cycle progression, apoptosis (programmed cell death), receptor-mediated endocytosis, and signal transduction. Imbalances or dysfunctions within this system can lead to pathological conditions. Apart from this, disruptions in ubiquitin pathways can also contribute to the development of human diseases, including neurodegenerative conditions and tumors.
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
物理的形状
In 100 mM NaCl, 50 mM HEPES, pH 8.0.
再構成
Following initial thaw, aliquot and freeze (-70°C).
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
662057-MG:
662057-1MG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Metalloregulation of Protein Clearance: New Therapeutic Avenues for Neurodegenerative Diseases
Munoz ET, et al.
Abstr. Soc. Neurosci (2017)
Ubiquitin
Kona F.R, et al.
Brenner and Rector's The Kidney E-book, 242-245 (2013)
Ubiquitin networks in cancer
Kirkin V and Dikic I
Current Opinion in Genetics & Development, 21(1), 21-28 (2011)
Mengmeng Niu et al.
PLoS biology, 19(2), e3001113-e3001113 (2021-02-25)
Transforming growth factor-β (TGF-β) signaling plays a critical role in promoting epithelial-to-mesenchymal transition (EMT), cell migration, invasion, and tumor metastasis. ΔNp63α, the major isoform of p63 protein expressed in epithelial cells, is a key transcriptional regulator of cell adhesion program
Mengmeng Niu et al.
Nature communications, 12(1), 5919-5919 (2021-10-13)
Abnormal activation of epidermal growth factor receptor (EGFR) drives non-small cell lung cancer (NSCLC) development. EGFR mutations-mediated resistance to tyrosine-kinase inhibitors (TKIs) is a major hurdle for NSCLC treatment. Here, we show that F-box protein FBXL2 targets EGFR and EGFR
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