495609

Okadaic Acid, Prorocentrum sp.

InSolution, ≥95%, inhibitor of protein phosphatase 1

• 別名: InSolution Okadaic Acid, Prorocentrum sp.
• 実験式（ヒル表記法）: C₄₄H₆₈O₁₃
• 分子量: 805.00
• UNSPSCコード: 12352106

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

An ionophore-like polyether derivative of a C₃₈ fatty acid compound isolated from dinoflagellates that have fed on the marine sponge Halinchrondria okadai. It is a potent non-comepetitive, reversible inhibitor of serine/threonine-specific protein phosphatases 1 (PP1, IC₅₀ = 10-15 nM, rabbit skeletal muscle, catalytic subunit) and 2A (PP2A, IC₅₀ = 0.1 nM, rabbit skeletal muscle, catalytic subunit). It has only a trivial effect on protein phosphatase 2B (IC₅₀ = 5 µM), a Ca²⁺/calmodulin-dependent enzyme, while PP2C, a Mg²⁺ dependent enzyme, is unaffected. Okadaic acid has no significant effect on the activities of tyrosine phosphatases, alkaline phosphatase, acid phosphatase, and inositol trisphosphatase. It is also a non-phorbol ester-type tumor promoter on mouse skin. Useful for the study of protein phosphatases and of PP1/PP2A in cell extracts, as well as in intact cells. Induces apoptosis in human breast carcinoma (MB-231 and MCF-7) and in myeloid cells, but inhibits glucocorticoid-induced apoptosis in T-cell hybridomas. Has marked contractile effect on smooth muscles and heart muscles. Implicated as a causative agent of diarrhetic shellfish poisoning.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
protein phosphatase

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 10-15 nM against protein phosphatase 1; 0.1 nM against protein phosphatase 2A

包装

Packaged under inert gas

警告

Toxicity: Toxic (F)

物理的形状

A 250 µM (25 µg/124 µl) solution of Okadaic Acid, (Cat. No. 495604) in DMSO.

再構成

Following initial thaw, aliquot and freeze (-20°C).

その他情報

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

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法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• 引火点(℃): 87 °C - closed cup - (Dimethylsulfoxide)