382159-M

PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

liquid, Calbiochem^®

• UNSPSCコード: 12352203

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: liquid
• 含みます: ≤0.1% sodium azide as preservative
• 交差性: Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• アイソタイプ: IgG
• 保管温度: −20°C
• ターゲットの翻訳後修飾: phosphorylation (pSer10)
• 遺伝子情報: human ... H3C1(8350)

詳細

詳細

Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

This PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).

免疫原

Human

a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH

アプリケーション

Immunoblotting (0.5 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunohistochemistry (see comments)

Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)

警告

Toxicity: Standard Handling (A)

物理的形状

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

再構成

Following initial thaw, aliquot and freeze (-20°C).

アナリシスノート

Positive Control
Acid-extracted proteins from colcemid-arrested HeLa cells

その他情報

Ajiro, K., et al. 1996. J. Biol. Chem.271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.

For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable